Home » PP2A alpha » PP2A alpha Antibodies » PP2A alpha Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

PP2A alpha Antibody [Unconjugated]

 (2 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of U937 human histiocytic lymphoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse/Rat PP2A Catalytic Subunit, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1653) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PP2A Catalytic Subunit, at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of U937 human histiocytic lymphoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse/Rat PP2A Catalytic ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1653-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

PP2A alpha Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PP2A-alpha catalytic subunit
Trp209-Leu309
Accession # P67775
Specificity
Detects endogenous human, mouse, and rat PP2A in Western blots. Because PP2A-alpha and PP2A-beta catalytic subunits are identical in the region used for the antigen, it is expected that both isoforms will be detected by the antibody.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP2CA
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1653 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP2A alpha Antibody [Unconjugated]

  • EC 3.1.3.16
  • PP2A
  • PP2A-alpha
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • PPP2CA
  • protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
  • protein phosphatase 2, catalytic subunit, alpha isozyme
  • protein phosphatase 2A catalytic subunit, alpha isoform
  • Replication protein C
  • RP-C
  • serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
  • serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Background

Protein Phosphatase 2A (PP2A) is an enzyme that removes phosphates covalently attached to serine and threonine residues in proteins. It contains three subunits; a catalytic subunit C (PP2Ac), an assembly/structural subunit A (PP2Aa), and a regulatory subunit B (PP2Ab). PP2A is a ubiquitous enzyme that plays a role in regulating many cellular activities varying from ion transport in erythrocytes to suppressing tumor growth. Binding of the SV40 small T antigen to the PP2A phosphatase complex is known to cause oncogenic transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

PCNA Antibody
(PC10)
NB500-106
Immunocytochemistry/ Immunofluorescence PCNA Antibody (PC10)
Western Blot PCNA Antibody (PC10)
Flow Cytometry PCNA Antibody (PC10)
(5 Reviews)
(46 Publications)
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
RFC1 Antibody
[Unconjugated]
AF6457
Western Blot RFC1 Antibody [Unconjugated]
Species: Hu
Applications: WB
Reduced Folate
Carrier/SLC19A1 Antib
NBP1-59904
Immunohistochemistry-Paraffin Reduced Folate Carrier/SLC19A1 Antibody
Western Blot Reduced Folate Carrier/SLC19A1 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Bcl-2 related
protein A1 Antibody ( ...
NBP2-67563
Flow Cytometry Bcl-2 related protein A1 Antibody (SC05-42)
Immunohistochemistry-Paraffin Bcl-2 related protein A1 Antibody (SC05-42)
Immunohistochemistry-Paraffin Bcl-2 related protein A1 Antibody (SC05-42)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
MAT1A Antibody
NBP3-13066
Immunohistochemistry-Paraffin MAT1A Antibody
Immunohistochemistry-Paraffin MAT1A Antibody
Western Blot MAT1A Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
PPP2R4 Antibody
(529245)
MAB5929
Western Blot PPP2R4 Antibody (529245)
Species: Hu, Mu, Rt
Applications: WB
RFC2 Antibody
(OTI2B2)
NBP2-45570
Flow Cytometry RFC2 Antibody (OTI2B2)
Immunohistochemistry RFC2 Antibody (OTI2B2)
Immunohistochemistry RFC2 Antibody (OTI2B2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
RFC4 Antibody
(OTI1A8)
NBP2-45946
Immunocytochemistry/ Immunofluorescence RFC4 Antibody (OTI1A8)
Immunohistochemistry RFC4 Antibody (OTI1A8)
Immunohistochemistry RFC4 Antibody (OTI1A8)
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Inorganic
Pyrophosphatase/PPA1
NBP1-31348
Immunocytochemistry/ Immunofluorescence Inorganic Pyrophosphatase/PPA1 Antibody
Western Blot Inorganic Pyrophosphatase/PPA1 Antibody
Western Blot Inorganic Pyrophosphatase/PPA1 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
ATP6V0A1 Antibody
NBP1-89342
Immunocytochemistry/ Immunofluorescence ATP6V0A1 Antibody
Immunohistochemistry-Paraffin ATP6V0A1 Antibody
Immunohistochemistry-Paraffin ATP6V0A1 Antibody
(1 Review)
(13 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Rad17 [p Ser645]
Antibody
NBP2-24457
Western Blot Rad17 [p Ser645] Antibody
Immunohistochemistry-Paraffin Rad17 [p Ser645] Antibody
Western Blot Rad17 [p Ser645] Antibody
Species: Hu, Mu, Pm
Applications: IHC, IHC-P, WB
c-Fos Antibody
(2H2)
NBP2-50037
Western Blot c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
(1 Review)
(20 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(50 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Bcl-xL Antibody
NB100-56104
Western Blot Bcl-xL Antibody
Immunohistochemistry-Paraffin Bcl-xL Antibody
Western Blot Bcl-xL Antibody
(1 Review)
(3 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
CHTF18 Antibody
(1F5)
H00063922-M01
Sandwich ELISA CHTF18 Antibody (1F5)
Western Blot CHTF18 Antibody (1F5)
Western Blot CHTF18 Antibody (1F5)
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
RFC3 Antibody
NBP1-82668
Immunohistochemistry-Paraffin RFC3 Antibody
Western Blot RFC3 Antibody
Immunocytochemistry/ Immunofluorescence RFC3 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for PP2A alpha Antibody (AF1653)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(1)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(1)
Rat
(1)
All Species

Reviews for PP2A alpha Antibody (AF1653) (0)

There are no reviews for PP2A alpha Antibody (AF1653). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PP2A alpha Antibody (AF1653) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PP2A alpha Products

Blogs on PP2A alpha

There are no specific blogs for PP2A alpha, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB58891, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
Rabbit IgG Isotype Control [Unconjugated]
AB-105-C
Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
PCNA Antibody (PC10)
NB500-106

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PP2A alpha Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol PPP2CA
Uniprot