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Human PP14/Glycodelin ELISA Kit (Colorimetric)

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ELISA: Human PP14/Glycodelin ELISA Kit (Colorimetric) [NBP2-82133] - Samples were spiked with high concentrations of Human PP14/Glycodelin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human PP14/Glycodelin ELISA Kit (Colorimetric) [NBP2-82133] - Samples were spiked with high concentrations of Human PP14/Glycodelin and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human PP14/Glycodelin ELISA Kit (Colorimetric) [NBP2-82133] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
4.69 - 300 ng/mL
Sensitivity
2.81 ng/mL

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Human PP14/Glycodelin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human PP14/Glycodelin in samples. No Significant cross-reactivity or interference between Human PP14/Glycodelin and analogues was observed.
Standard Curve Range
4.69 - 300 ng/mL
Sensitivity
2.81 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 6%
Spike Recovery
average = 96% for serum; average = 98% for EDTA plasma; average = 102% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PAEP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Human PP14/Glycodelin ELISA Kit (Colorimetric)

  • alpha uterine protein
  • GdF
  • GdS
  • Glycodelin
  • glycodelin-A
  • glycodelin-F
  • glycodelin-S
  • MGC138509
  • MGC142288
  • PAEP
  • PEP
  • Placental protein 14
  • PP14 protein (placental protein 14)
  • PP14
  • PP14GDPAEGGdA
  • pregnancy-associated endometrial a
  • Pregnancy-associated endometrial alpha-2 globulin
  • pregnancy-associated endometrial alpha-2-globulin
  • progestagen-associated endometrial protein (placental protein 14
  • progestagen-associated endometrial proteinPEG
  • Progesterone-associated endometrial protein

Background

PAEP (Progesterone-associated endometrial protein, glycodelin, PEG, PP14) is a glycoprotein belonging to lipocalin structural superfamily. It shares a sequence homology to beta-lactoglobulins containing a retinol-binding motif and it is mainly produced by secretory and decidualized endometrium in women and by seminal vesicle epithelium in men (1). PAEP function is not clearly understood. However, glycosylated version of PAEP (GdA) has been associated to contraceptive and immunosuppressive activities during reproduction. PAEP is the main protein produced by secretory endometrium during mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. Therefore, PAEP has been linked as an ideal biomarker of endometrial activity and function for in vitro fertilization treated women (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human PP14/Glycodelin ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol PAEP