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POLR1C Antibody

Images

 
Western Blot: POLR1C Antibody [NBP1-87114] - Analysis in control (vector only transfected HEK293T lysate) and POLR1C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: POLR1C Antibody [NBP1-87114] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

POLR1C Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:NNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHD
Predicted Species
Mouse (95%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR1C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
POLR1C Protein (NBP1-87114PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for POLR1C Antibody

  • AC40
  • DNA-directed RNA polymerase I subunit C
  • DNA-directed RNA polymerases I and III 40 kDa polypeptide
  • POLR1E
  • polymerase (RNA) I polypeptide C, 30kDa
  • RNA polymerase I subunit
  • RPA39RNA polymerases I and III subunit AC1
  • RPA40TCS3
  • RPA5RPAC1DNA-directed RNA polymerases I and III subunit RPAC1
  • RPC40

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for POLR1C Antibody (NBP1-87114) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POLR1C