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UBTF Antibody

Images

 
Genetic Strategies: Western Blot: UBTF Antibody [NBP1-82545] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: UBTF Antibody [NBP1-82545] - Staining of human cell line U-2 OS shows localization to nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse brain shows strong positivity in neurons in the CA1 and granular cell layers in the hippocampus.
Western Blot: UBTF Antibody [NBP1-82545] - Analysis in mouse cerebral cortex tissue.
Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse basal forebrain shows moderate nuclear positivity in neurons in the caudate putamen.
Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse brain shows strong nuclear positivity in neurons in the cerebral cortex.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

UBTF Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: WKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKK
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBTF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
UBTF Recombinant Protein Antigen (NBP1-82545PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-82545 in the following applications:

Publications
Read Publications using
NBP1-82545 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UBTF Antibody

  • 90-KDa Nucleolus Organizer Region Autoantigen
  • Autoantigen NOR-90
  • NOR-90
  • nucleolar transcription factor 1
  • UBF
  • UBF1
  • UBF-1
  • UBF2
  • UBFUBF-1
  • upstream binding transcription factor, RNA polymerase I
  • Upstream-binding factor 1

Background

Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UBTF Antibody (NBP1-82545)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(2)
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Mouse
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Review for UBTF Antibody (NBP1-82545) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-82545:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot UBTF NBP1-82545
Enlarge
3
reviewed by:
Ashwini Katre
WB Human 07/24/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman ovarian carcinoma cell lines
SpeciesHuman
LotA79395

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UBTF Antibody (NBP1-82545) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

3
5
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3
1
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1
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Ashwini Katre
07/24/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol UBTF