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CDK5RAP3 Antibody (1G4)

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Western Blot: CDK5RAP3 Antibody (1G4) [H00080279-M01] - CDK5RAP3 monoclonal antibody (M01), clone 1G4 Analysis of CDK5RAP3 expression in A-431.
Western Blot: CDK5RAP3 Antibody (1G4) [H00080279-M01] - CDK5RAP3 monoclonal antibody (M01), clone 1G4 Analysis of CDK5RAP3 expression in A-431.
Immunocytochemistry/ Immunofluorescence: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Analysis of monoclonal antibody to CDK5RAP3 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Analysis of monoclonal antibody to CDK5RAP3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Detection limit for recombinant GST tagged CDK5RAP3 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CDK5RAP3 Antibody (1G4) Summary

Immunogen
CDK5RAP3 (AAH09957, 1 a.a. ~ 506 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEDHQHVPIDIQTSKLLDWLVDRRHCSLKWQSLVLTIREKINAAIQDMPESEEIAQLLSGSYIHYFHCLRILDLLKGTEASTKNIFGRYSSQRMKDWQEIIALYEKDNTYLVELSSLLVRNVNYEIPSLKKQIAKCQQLQQEYSRKEEECQAGAAEMREQFYHSCKQYGITGENVRGELLALVKDLPSQLAEIGAAAQQSLGEAIDVYQASVGFVCESPTEQVLPMLRFVQKRGNSTVYEWRTGTEPSVVERPHLEELPEQVAEDAIDWGDFGVEAVSEGTDSGISAEAAGIDWGIFPESDSKDPGGDGIDWGDDAVALQITVLEAGTQAPEGVARGPDALTLLEYTETRNQFLDELMELEIFLAQRAVELSEEADVLSVSQFQLAPAILQGQTKEKMVTMVSVLEDLIGKLTSLQLQHLFMILASPRYVDRVTEFLQQKLKQSQLLALKKELMVQKQQEALEEQAALEPKLDLLLEKTKELQKLIEADISKRYSGRPVNLMGTSL
Specificity
CDK5RAP3 - CDK5 regulatory subunit associated protein 3
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDK5RAP3
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publications using
H00080279-M01 in the following applications:

  • B/N
    1 publication
  • WB
    4 publications

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CDK5RAP3 Antibody (1G4)

  • C53
  • CDK5 activator-binding protein C53
  • CDK5 regulatory subunit associated protein 3
  • FLJ13660
  • HSF-27
  • IC53CDK5 regulatory subunit-associated protein 3
  • ischemic heart CDK5 activator-binding protein C53
  • LXXLL/leucine-zipper-containing ARFbinding protein
  • LZAP
  • MST016
  • OK/SW-cl.114
  • Protein HSF-27

Background

Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein, which may be a substrate of neuronal CDC2-like kinase, has also been found in vascular endothelial cells, where it mediates cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDK5RAP3 Antibody (H00080279-M01)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: B/N, WB.


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(4)
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Bioinformatics

Gene Symbol CDK5RAP3
Entrez
OMIM
Uniprot