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PLK1 Antibody (AZ44) - BSA Free

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Product Details

Summary
Reactivity XpSpecies Glossary
Applications WB, ELISA
Clone
AZ44
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2747AF350
Alexa Fluor 405
NB100-2747AF405
Alexa Fluor 488
NB100-2747AF488
Alexa Fluor 532
NB100-2747AF532
Alexa Fluor 594
NB100-2747AF594
Alexa Fluor 647
NB100-2747AF647
Alexa Fluor 700
NB100-2747AF700
Alexa Fluor 750
NB100-2747AF750
Biotin
NB100-2747B
DyLight 350
NB100-2747UV
DyLight 405
NB100-2747V
DyLight 488
NB100-2747G
DyLight 550
NB100-2747R
DyLight 594
NB100-2747DL594
DyLight 650
NB100-2747C
DyLight 680
NB100-2747FR
DyLight 755
NB100-2747IR
FITC
NB100-2747F
HRP
NB100-2747H
Janelia Fluor 549
NB100-2747JF549
Janelia Fluor 646
NB100-2747JF646
mFluor Violet 450 SE
NB100-2747MFV450
mFluor Violet 500 SE
NB100-2747MFV500
mFluor Violet 610 SE
NB100-2747MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NB100-2747-0.025mg
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PLK1 Antibody (AZ44) - BSA Free Summary

Immunogen
Baculo-expressed PLK1 from Xenopus laevis
Epitope
This antibody is thought to bind to a phosphorylated epitope. However, this has not yet been proved.
Localization
Nuclear
Marker
Mitosis Marker
Specificity
Plx is activated by phosphorylation. AZ44 only detects the mitotic form of Plx (Xenopus laevis) which may be due to the recognition of a phosphorylated epitope. Recommended concentration: 1 ug/ml
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PLK1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Western Blot 1:100 - 1:2000
Application Notes
Positive control(s): Xenopus egg extract.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for PLK1 Antibody (AZ44) - BSA Free

  • cell cycle regulated protein kinase
  • EC 2.7.11
  • EC 2.7.11.21
  • PLK
  • PLK1
  • PLK-1
  • polo (Drosophia)-like kinase
  • polo like kinase
  • polo-like kinase 1polo-like kinase (Drosophila)
  • Serine/threonine-protein kinase 13
  • serine/threonine-protein kinase PLK1
  • STPK13

Background

Polo-like kinase (PLK) plays an important role in cell cycle regulation. It is required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a polo-like kinase kinase (PLKK) that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

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