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PlGF-2 Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF465
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Catalog# & Formulation Size Price
Carrier Free
AF465-SP
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PlGF-2 Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2 (R&D Systems, Catalog # 465-PL)
Ala24-Pro158
Accession # Q544A5
Specificity
Detects mouse PlGF-2 in direct ELISAs and Western blots. In these formats, less than 5% cross‑reactivity with recombinant human PlGF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Pgf
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF465 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PlGF-2 Antibody

  • OORS
  • PlGF2
  • PlGF-2

Background

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high-affinity to Flt-1, but not to Flk-1/KDR.

  1. DiPalma, T. et al. (1996) Mamm. Genome 7:6.
  2. Cao, Y. et al. (1997) Biochem. Biophys. Res. Commun. 235:493.
  3. Ferrara, N. et al. (1997) Endocrin. Rev. 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF465
PlGF-2 Antibody
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Publications for PlGF-2 Antibody (AF465)(4)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: IHC, Immunohistochemistry, Western Blot.


Filter By Application
IHC
(1)
Immunohistochemistry
(1)
Western Blot
(2)
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Mouse
(3)
Rat
(1)
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Showing Publications 1 - 4 of 4.
Publications using AF465 Applications Species
Tai, Y;Chow, A;Han, S;Coker, C;Ma, W;Gu, Y;Estrada Navarro, V;Kandpal, M;Hibshoosh, H;Kalinsky, K;Manova-Todorova, K;Safonov, A;Walsh, EM;Robson, M;Norton, L;Baer, R;Merghoub, T;Biswas, AK;Acharyya, S; FLT1 activation in cancer cells promotes PARP-inhibitor resistance in breast cancer EMBO molecular medicine 2024-07-02 [PMID: 38956205] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Iwama H, Uemura S, Naya N, Imagawa K, Takemoto Y, Asai O, Onoue K, Okayama S, Somekawa S, Kida Y, Takeda Y, Nakatani K, Takaoka M, Kawata H, Horii M, Nakajima T, Doi N, Saito Y Cardiac expression of placental growth factor predicts the improvement of chronic phase left ventricular function in patients with acute myocardial infarction. J. Am. Coll. Cardiol., 2006-03-27;47(8):1559-67. 2006-03-27 [PMID: 16630991] (IHC, Mouse) IHC Mouse
Tsao PN, Su YN, Li H, Huang PH, Chien CT, Lai YL, Lee CN, Chen CA, Cheng WF, Wei SC, Yu CJ, Hsieh FJ, Hsu SM Overexpression of placenta growth factor contributes to the pathogenesis of pulmonary emphysema. Am. J. Respir. Crit. Care Med., 2003-11-25;169(4):505-11. 2003-11-25 [PMID: 14644931] (Western Blot, Mouse) Western Blot Mouse
Maynard SE, Min JY, Merchan J, Lim KH, Mondal S, Stillman IE, Epstein FH, Karumanchi SA Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia. J. Clin. Invest., 2003-03-01;111(5):649-58. 2003-03-01 [PMID: 12618519] (Western Blot, Rat) Western Blot Rat

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Bioinformatics

Gene Symbol Pgf
Uniprot