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PlGF-2 Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF465
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF465
Carrier Free
AF465
Carrier Free
AF465-SP
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PlGF-2 Antibody [Biotin] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2 (R&D Systems, Catalog # 465-PL)
Ala24-Pro158
Accession # Q544A5
Specificity
Detects mouse PlGF-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with rmVEGF R1 Fc Chimera, rmVEGF R2 Fc Chimera, and rmVEGF R3 Fc Chimera is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Pgf
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse PlGF-2 Antibody (Catalog # MAB4651)
ELISA Detection: Mouse PlGF-2 Biotinylated Antibody (Catalog # BAF465)
Standard: Recombinant Mouse PlGF-2 (Catalog # 465-PL)
Readout System
Publications
Read Publications using
BAF465 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PlGF-2 Antibody [Biotin]

  • OORS
  • PlGF2
  • PlGF-2

Background

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high-affinity to Flt-1, but not to Flk-1/KDR.

  1. DiPalma, T. et al. (1996) Mamm. Genome 7:6.
  2. Cao, Y. et al. (1997) Biochem. Biophys. Res. Commun. 235:493.
  3. Ferrara, N. et al. (1997) Endocrin. Rev. 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PlGF-2 Antibody (BAF465)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ELISA Detection, ELISA Development.


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Bioinformatics

Gene Symbol Pgf
Uniprot