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Semaphorin 6A Antibody [Unconjugated]

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Semaphorin 6A was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Semaphorin 6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1615) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Semaphorin 6A was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Semaphorin 6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1615) at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1615
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1615-SP
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Semaphorin 6A Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464
Specificity
Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Semaphorin 6A is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3F, and rmSemaphorin 7A is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SEMA6A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1615 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 6A Antibody [Unconjugated]

  • KIAA1368HT018
  • sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
  • Sema VIA
  • SEMA
  • Sema6A
  • SEMA6A1
  • SEMA6A-1
  • Semaphorin 6A
  • semaphorin 6A-1
  • Semaphorin VIA
  • semaphorin-6A
  • semaphorin-6A-1
  • SEMAQ
  • VIA

Background

Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal (1).

  1. Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Semaphorin 6A Antibody (AF1615)(7)

We have publications tested in 3 confirmed species: Mouse, Primate - Chlorocebus pygerythrus (Vervet Monkey), Transgenic Mouse.

We have publications tested in 4 applications: ICC, IHC, Immunohistochemistry, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
Immunohistochemistry
(1)
Western Blot
(1)
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Mouse
(3)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
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(1)
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Showing Publications 1 - 7 of 7.
Publications using AF1615 Applications Species
Lettieri, A;Oleari, R;van den Munkhof, MH;van Battum, EY;Verhagen, MG;Tacconi, C;Spreafico, M;Paganoni, AJJ;Azzarelli, R;Andre', V;Amoruso, F;Palazzolo, L;Eberini, I;Dunkel, L;Howard, SR;Fantin, A;Pasterkamp, RJ;Cariboni, A; SEMA6A drives GnRH neuron-dependent puberty onset by tuning median eminence vascular permeability Nature communications 2023-12-07 [PMID: 38062045] (Immunohistochemistry, Mouse, Transgenic Mouse) Immunohistochemistry Mouse, Transgenic Mouse
Van Battum E, Heitz-Marchaland C, Zagar Y Et al. Plexin-B2 controls the timing of differentiation and the motility of cerebellar granule neurons eLife 6/8/2021 [PMID: 34100719]
Hunsang Lee, Greg L. Beilhartz, Iga Kucharska, Swetha Raman, Hong Cui, Mandy Hiu Yi Lam et al. Recognition of Semaphorin Proteins by P. sordellii Lethal Toxin Reveals Principles of Receptor Specificity in Clostridial Toxins Cell 2020-07-01 [PMID: 32589945]
Wang J, Tao J, Chen D, Cai J, Irani K, Wang Q, Yuan H, Chen A MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus. Arterioscler Thromb Vasc Biol, 2013-10-31;34(0):99. 2013-10-31 [PMID: 24177325] (Western Blot, Mouse) Western Blot Mouse
Segarra , Marta, Ohnuki , Hidetaka, Maric , Dragan, Salvucci , Ombretta, Hou , Xu, Kumar , Anil, Li , Xuri, Tosato , Giovanna Semaphorin 6A regulates angiogenesis by modulating VEGF signaling. Blood, 2012-09-24;120(19):4104-15. 2012-09-24 [PMID: 23007403] (IHC, Mouse) IHC Mouse
Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, D'Hooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chedotal A, Honnorat J, Thomasset N, Carmeliet P, Meissirel C Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1. J. Neurosci., 2010-11-10;30(45):15052-66. 11/10/2010 [PMID: 21068311]
Haklai-Topper L, Mlechkovich G, Savariego D Cis interaction between Semaphorin6A and Plexin-A4 modulates the repulsive response to Sema6A. EMBO J., 2010-07-06;29(15):2635-45. 2010-07-06 [PMID: 20606624] (ICC, Primate - Chlorocebus pygerythrus (Vervet Monkey)) ICC Primate - Chlorocebus pygerythrus (Vervet Monkey)

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Bioinformatics

Gene Symbol SEMA6A
Uniprot