![Western Blot: PLA2R1 Antibody (12-6-5) [NBP2-50248] - 1ug of recombinant full length extracellular domain of human PLA2R (NC8) and a N-terminus fragment of PLA2R (NC3) were subjected to SDS PAGE followed by western blot using Ms anti-PLAR (Cl12-6-5) at dilution 1:10000.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Western-Blot-NBP2-50248-img0001.jpg "Western Blot: PLA2R1 Antibody (12-6-5) [NBP2-50248] - 1ug of recombinant full length extracellular domain of human PLA2R (NC8) and a N-terminus fragment of PLA2R (NC3) were subjected to SDS PAGE followed by western blot using Ms anti-PLAR (Cl12-6-5) at dilution 1:10000.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 12-6-5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3). Genbank acc no. U17033; amino acids 20-663 Recombinant protein expressed in HEK 293-EBNA-1 cells |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PLA2R1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Western Blot PLA2G2A Antibody [Unconjugated]](https://images.novusbio.com/images2/PLA2G2A_AF4925_Western_Blot_10430.jpg)
![Immunohistochemistry PLA2G2A Antibody [Unconjugated]](https://images.novusbio.com/images2/PLA2G2A_AF4925_Immunohistochemistry_10959.jpg)
![Simple Western PLA2G2A Antibody [Unconjugated]](https://images.novusbio.com/images2/PLA2G2A_AF4925_Simple_Western_17643.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PLA2R1 |
![Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Staining of PLA2R over-expressing podocytes revealing cell surface expression of the PLA2R receptor. Dilution 1:400 (under 40x lens).](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunocytochemistry-Immunofluorescence-NBP2-50248-img0002.jpg "Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Staining of PLA2R over-expressing podocytes revealing cell surface expression of the PLA2R receptor. Dilution 1:400 (under 40x lens).")
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Membranous nephropathy kidney showing staining of PLA2R in the capillary wall of the glomerular basement membrane. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0006.jpg "Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Membranous nephropathy kidney showing staining of PLA2R in the capillary wall of the glomerular basement membrane. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.")
![Flow Cytometry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - A surface stain was performed on HeLa cells with PLA2R1 Antibody (12-6-5) NBP2-50248PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Flow-Cytometry-NBP2-50248-img0007.jpg "Flow Cytometry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - A surface stain was performed on HeLa cells with PLA2R1 Antibody (12-6-5) NBP2-50248PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.")
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Normal human kidney showing glomerular specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0004.jpg "Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Normal human kidney showing glomerular specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.")
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Normal human kidney showing podocyte specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0005.jpg "Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Normal human kidney showing podocyte specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.")
![Flow Cytometry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Overlay flow cytometry histograms of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) stained with Ms Cl12-6-5) at dilution 1:200. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line).](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Flow-Cytometry-NBP2-50248-img0003.jpg "Flow Cytometry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Overlay flow cytometry histograms of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) stained with Ms Cl12-6-5) at dilution 1:200. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line).")
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