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![Western Blot: PLA2R1 Antibody (12-6-5) [NBP2-50248] - 1ug of recombinant full length extracellular domain of human PLA2R (NC8) and a N-terminus fragment of PLA2R (NC3) were subjected to SDS PAGE followed by western blot using Ms anti-PLAR (Cl12-6-5) at dilution 1:10000.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Western-Blot-NBP2-50248-img0001.jpg "Western Blot: PLA2R1 Antibody (12-6-5) [NBP2-50248] - 1ug of recombinant full length extracellular domain of human PLA2R (NC8) and a N-terminus fragment of PLA2R (NC3) were subjected to SDS PAGE followed by western blot using Ms anti-PLAR (Cl12-6-5) at dilution 1:10000.")
![Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Staining of PLA2R over-expressing podocytes revealing cell surface expression of the PLA2R receptor. Dilution 1:400 (under 40x lens).](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunocytochemistry-Immunofluorescence-NBP2-50248-img0002.jpg)
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Membranous nephropathy kidney showing staining of PLA2R in the capillary wall of the glomerular basement membrane. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0006.jpg)
![Flow Cytometry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - A surface stain was performed on HeLa cells with PLA2R1 Antibody (12-6-5) NBP2-50248PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Flow-Cytometry-NBP2-50248-img0007.jpg)
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Normal human kidney showing glomerular specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0004.jpg)
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Normal human kidney showing podocyte specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0005.jpg)
![Flow Cytometry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Overlay flow cytometry histograms of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) stained with Ms Cl12-6-5) at dilution 1:200. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line).](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Flow-Cytometry-NBP2-50248-img0003.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining in human kidney and pancreas tissues . Corresponding PLA2R1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-84449-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining in human kidney and pancreas tissues . Corresponding PLA2R1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PLA2R1 Antibody [NBP1-84449] - Analysis in control (vector only transfected HEK293T lysate) and PLA2R1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PLA2R1-Antibody-Western-Blot-NBP1-84449-img0011.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining o human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-84449-img0012.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-84449-img0004.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining of human kidney shows weak membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-84449-img0008.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933] - Staining in human kidney and pancreas tissues. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0474-Immunohistochemistry-Paraffin-NBP2-52933-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933] - Staining in human kidney and pancreas tissues. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PLA2R1 Antibody (CL0474) [NBP2-52933] - Analysis in human kidney tissue.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0474-Western-Blot-NBP2-52933-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP2-52933] - Enhanced validation of the Anti-PLA2R1 monoclonal antibody by comparing the immunofluorescence staining in human cell lines Hela and MCF7 exhibiting a relatively high and low expression of PLA2R1 (based on RNA-seq values), respectively. The PLA2R1 (green signal) is present in Hela cells but is absent in MCF7 cells. The anti-HDAC1 antibody was used as a loading control (red signal). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0474-Immunocytochemistry-Immunofluorescence-NBP2-52933-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP2-52933] - Staining of HeLa cells showing specific staining in the cytosol and plasma membrane in green. Microtubule- and nuclear probes are visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0474-Immunocytochemistry-Immunofluorescence-NBP2-52933-img0013.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933] - Staining of human kidney with membranous nephropathy shows strong membranous immunoreactivity in renal glomeruli.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0474-Immunohistochemistry-Paraffin-NBP2-52933-img0001.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0474-Immunohistochemistry-Paraffin-NBP2-52933-img0004.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933] - Staining of human kidney shows weak membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0474-Immunohistochemistry-Paraffin-NBP2-52933-img0010.jpg)
![Flow Cytometry: PLA2R1 Antibody [NBP1-46116] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-Flow-Cytometry-NBP1-46116-img0001.jpg "Flow Cytometry: PLA2R1 Antibody [NBP1-46116] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: PLA2R1 Antibody (CL0485) [NBP2-52934] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: PLA2R1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0485-Western-Blot-NBP2-52934-img0003.jpg "Western Blot: PLA2R1 Antibody (CL0485) [NBP2-52934] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: PLA2R1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)")
![Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0485) [NBP2-52934] - Staining of normal human kidney shows absence of positivity.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0485-Immunohistochemistry-Paraffin-NBP2-52934-img0002.jpg)
![Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0485) [NBP2-52934] - Staining of human kidney with membranous nephropathy shows strong membranous immunoreactivity in renal glomeruli.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-CL0485-Immunohistochemistry-Paraffin-NBP2-52934-img0001.jpg)
![Western Blot: PLA2R1 Overexpression Lysate (Adult Normal) [NBP2-08815] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLA2R1.](http://images.novusbio.com/fullsize/PLA2R1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08815-img0001.jpg "Western Blot: PLA2R1 Overexpression Lysate (Adult Normal) [NBP2-08815] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLA2R1.")
![ELISA: Human PLA2R1 ELISA Kit (Colorimetric) [NBP3-39348] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39348_human-pla2r1-elisa-kit-colorimetric-2110202415264375.png "ELISA: Human PLA2R1 ELISA Kit (Colorimetric) [NBP3-39348] - Standard Curve Reference")
![ELISA: Human PLA2R1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39349] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39349_human-pla2r1-ready-to-use-elisa-kit-colorimetric-21102024153806.png "ELISA: Human PLA2R1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39349] - Standard Curve Reference")