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PKM2 Antibody [HRP]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, WB, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-48308AF350
Alexa Fluor 405
NBP1-48308AF405
Alexa Fluor 488
NBP1-48308AF488
Alexa Fluor 532
NBP1-48308AF532
Alexa Fluor 594
NBP1-48308AF594
Alexa Fluor 647
NBP1-48308AF647
Alexa Fluor 700
NBP1-48308AF700
Alexa Fluor 750
NBP1-48308AF750
Allophycocyanin
NBP1-48308APC
Biotin
NBP1-48308B
CoraFluor 1
NBP1-48308CL1
DyLight 350
NBP1-48308UV
DyLight 405
NBP1-48308V
DyLight 488
NBP1-48308G
DyLight 550
NBP1-48308R
DyLight 594
NBP1-48308DL594
DyLight 650
NBP1-48308C
DyLight 680
NBP1-48308FR
DyLight 755
NBP1-48308IR
FITC
NBP1-48308F
Janelia Fluor 525
NBP1-48308JF525
Janelia Fluor 549
NBP1-48308JF549
Janelia Fluor 585
NBP1-48308JF585
Janelia Fluor 635
NBP1-48308JF635
Janelia Fluor 646
NBP1-48308JF646
Janelia Fluor 669
NBP1-48308JF669
PE
NBP1-48308PE
PerCP
NBP1-48308PCP
Unconjugated
NBP1-48308
mFluor Violet 450 SE
NBP1-48308MFV450
mFluor Violet 500 SE
NBP1-48308MFV500
mFluor Violet 610 SE
NBP1-48308MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-48308
BSA Free
NBP1-48308SS
BSA Free
NBP1-48308
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PKM2 Antibody [HRP] Summary

Immunogen
A synthetic peptide made to the internal region of human PKM2 protein (within residues 350-450). [Swiss-Prot: P14618]
Localization
Cytoplasm. Nucleus. Note: Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKM
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25416385).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for PKM2 Antibody [HRP]

  • CTHBP
  • Cytosolic thyroid hormone-binding protein
  • MGC3932
  • OIP3
  • OIP-3
  • OIP3EC 2.7.1.40
  • Opa-interacting protein 3
  • p58
  • PK, muscle type
  • PK2
  • PK3
  • PK3PKM
  • PKM2
  • Pyruvate kinase 2/3
  • pyruvate kinase isozymes M1/M2
  • Pyruvate kinase muscle isozyme
  • pyruvate kinase, muscle
  • TCB
  • THBP1
  • THBP1p58
  • Thyroid hormone-binding protein 1
  • thyroid hormone-binding protein, cytosolic
  • Tumor M2-PK

Background

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PKM