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PKHD1 Antibody (8G12A1)

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Flow Cytometry: PKHD1 Antibody (8G12A1) [NBP3-27118] - Analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
Flow Cytometry: PKHD1 Antibody (8G12A1) [NBP3-27118] - Analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
Immunohistochemistry: PKHD1 Antibody (8G12A1) [NBP3-27118] - Analysis of paraffin-embedded esophageal cancer tissues using PKHD1 Rat mAb with DAB staining.
ELISA: PKHD1 Antibody (8G12A1) [NBP3-27118] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Western Blot: PKHD1 Antibody (8G12A1) [NBP3-27118] - Analysis using PKHD1 mAb against mouse PKHD1 (AA: 3878-4060) recombinant protein. (Expected MW is 23 kDa)
Immunocytochemistry/ Immunofluorescence: PKHD1 Antibody (8G12A1) [NBP3-27118] - Analysis of A431 cells using PKHD1 Rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC
Clone
8G12A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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PKHD1 Antibody (8G12A1) Summary

Immunogen
Purified recombinant fragment of mouse PKHD1 (AA: 3878-4060) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PKHD1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Theoretical MW
445 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PKHD1 Antibody (8G12A1)

  • polycystic kidney and hepatic disease 1 (autosomal recessive)

Background

Found mainly in the cytoplasm and plasma membrane, PKHD1 plays an important role in protein binding and receptor activity. The protein encoded by PKHD1 has a single transmembrane (TM) spanning domain and various copies of an immunoglobulin-like plexin-transcription-factor domain. More specifically, these transcripts encode products which lack the TM and may be secreted. In humans, PKHD1 is highly expressed in the kidney by acting as a receptor protein in the cortical and medullary collecting ducts. Diseases associated with PKHD1 include polycystic kidney and hepatic disease, liver disease, neoplasia, fibrosis, cancer, adenocarcinoma and prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PKHD1