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PRKCSH Antibody

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Genetic Strategies: Western Blot: PRKCSH Antibody [NBP1-32006] - Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH ...read more
Immunocytochemistry/ Immunofluorescence: PRKCSH Antibody [NBP1-32006] - PRKCSH antibody detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde ...read more
Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP1-32006] - Normal liver.
Western Blot: PRKCSH Antibody [NBP1-32006] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: PRKCSH Antibody [NBP1-32006] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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PRKCSH Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PRKCSH. The exact sequence is proprietary.
Localization
Endoplasmic reticulum
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCSH
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:5000-1:20000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PRKCSH Antibody

  • AGE-R2,80K-H protein
  • G19P1AGE-binding receptor 2
  • glucosidase 2 subunit beta
  • Glucosidase II subunit beta
  • glucosidase II, beta subunit
  • hepatocystin
  • PCLD
  • PKCSH
  • PLD1
  • Protein kinase C substrate 60.1 kDa protein heavy chain
  • protein kinase C substrate 80K-H
  • protein kinase C substrate, 80 Kda protein

Background

This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKCSH