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PIK3C2A Antibody (OTI3H2)

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Western Blot: PIK3C2A Antibody (3H2) [NBP2-46395] - E.coli lysate (left lane) and E.coli lysate expressing human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A were separated by ...read more
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of HT29 cell lysate (35ug) by using PIK3C2A monoclonal antibody.
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI3H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PIK3C2A Antibody (OTI3H2) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PIK3C2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
190.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIK3C2A Antibody (OTI3H2)

  • C2-containing phosphatidylinositol kinase
  • CPK
  • DKFZp686L193
  • EC 2.7.1
  • EC 2.7.1.137
  • EC 2.7.1.153
  • EC 2.7.1.154
  • MGC142218
  • phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha
  • Phosphoinositide 3-kinase-C2-alpha
  • phosphoinositide-3-kinase, class 2, alpha polypeptide
  • PI3-K-C2(ALPHA)
  • PI3-K-C2A
  • PI3K-C2alpha
  • PI3K-C2-alpha
  • PIK3C2A
  • PtdIns-3-kinase C2 subunit alpha

Background

PIK3C2A belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIK3C2A