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PGK1 Antibody

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Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGK1 at approximately 43 to 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5455-SP
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PGK1 Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PGK1
Met1-Ile417
Accession # P00558
Specificity
Detects endogenous human, mouse and rat PGK1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PGK1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5455 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PGK1 Antibody

  • Cell migration-inducing gene 10 protein
  • EC 2.7.2.3
  • MGC142128
  • MGC8947
  • MIG10
  • PGK1
  • PGKA
  • PGKAMGC117307
  • phosphoglycerate kinase 1
  • Primer recognition protein 2
  • PRP 2
  • PRP2

Background

Phosphoglycerate kinase-1 (PGK1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1 alpha and is a potential biomarker and therapeutic target for cancer (4‑7).

  1. Beutler, E. (2002) Br. J. Haematol. 136:3.
  2. Flanagan, J.M. et al. (2006) Br. J. Haematol. 134:233.
  3. Svaasand, E.K. et al. (2007) Muscle Nerve. 36:579.
  4. Hwang, T.L. et al. (2006) Proteomics. 6:2259.
  5. Wang, J. et al. (2007) Cancer Res. 67:149.
  6. Zieker, D. et al. (2008) Cell Physiol. Biochem. 21:429.
  7. Jang, C.H. et al. (2008) Biosci. Biotechnol. Biochem. 72:1799.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PGK1 Antibody (AF5455)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol PGK1
Uniprot