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Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric)

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ELISA: Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric) [NBP3-39288] - Standard Curve Reference
ELISA: Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric) [NBP3-39288] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL (example only; lot dependent)
Sensitivity
13.5 pg/mL (example only; lot dependent)

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Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
31.25 - 2000 pg/mL (example only; lot dependent)
Sensitivity
13.5 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PAM

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric)

  • PAL
  • PAM
  • pancreatic peptidylglycine alpha-amidating monooxygenase
  • peptidyl alpha-amidating enzyme
  • peptidyl-alpha-hydroxyglycine alpha-amidating lyase
  • peptidylamidoglycolate lyase
  • peptidylglycine 2-hydroxylase
  • peptidyl-glycine alpha-amidating monooxygenase
  • peptidylglycine alpha-amidating monooxygenase
  • peptidylglycine alpha-hydroxylating monooxygenase
  • PHM

Background

PAM encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Manual - Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric)
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol PAM