Home » PDGF-B » PDGF-B Antibodies » PDGF-B Antibody (MM0014-5F66) - Azide and BSA Free
Novus Biologicals products are now on bio-techne.com

PDGF-B Antibody (MM0014-5F66) - Azide and BSA Free

 (2 Publications)
 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Immunohistochemistry-Paraffin: PDGFB Antibody (MM0014-5F66) [NB110-60980] - Human placenta (formalin fixed and paraffin embedded) section is subjected to IHC.
Immunohistochemistry-Paraffin: PDGFB Antibody (MM0014-5F66) [NB110-60980] - Human placenta (formalin fixed and paraffin embedded) section is subjected to IHC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
MM0014-5F66
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

PDGF-B Antibody (MM0014-5F66) - Azide and BSA Free Summary

Immunogen
Human recombinant PDGF-B
Specificity
This antibody shows approximately some degree (40%) cross-reactivity with recombinant PDGF-AB and no cross-reactivity with recombinant PDGF-AA.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDGFB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000
Application Notes
Flow Cytometry was reported in scientific literature. Proteinase K (20ug/ml in PBS) is recommended for antigen retrieval. This antibody is CyTOF ready.
Reviewed Applications
Read 1 Review rated 4
using
NB110-60980 in the following applications:
Publications
Read Publications using
NB110-60980 in the following applications:

  • FLOW
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for PDGF-B Antibody (MM0014-5F66) - Azide and BSA Free

  • B chain
  • Becaplermin
  • C-Sis
  • FLJ12858
  • IBGC5
  • PDGF subunit B
  • PDGF2
  • PDGF-2
  • PDGFB
  • PDGF-B
  • platelet-derived growth factor 2
  • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog)
  • Platelet-derived growth factor beta polypeptide
  • platelet-derived growth factor subunit B
  • platelet-derived growth factor, B chain
  • Platelet-derived growth factor, beta polypeptide (oncogene SIS)
  • Proto-oncogene c-Sis
  • SIS
  • SSV

Background

Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimmer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(685 Publications)
Species: Hu
Applications: ELISA
PDGF-AA
[Unconjugated]
221-AA
Bioactivity PDGF-AA [Unconjugated]
Cell Culture PDGF-AA [Unconjugated]
(57 Publications)
Species: Hu
Applications: BA
Collagen I Antibody
NB600-408
Immunohistochemistry-Paraffin Collagen I Antibody
Immunohistochemistry-Paraffin Collagen I Antibody
Immunohistochemistry-Paraffin Collagen I Antibody
(16 Reviews)
(322 Publications)
Species: Bv, Hu, Mu, Po, Rb, Rt
Applications: ELISA, FLISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, MiAr, PAGE, WB
PDGF-B Antibody
NBP1-58279
Western Blot PDGF-B Antibody
Immunocytochemistry/ Immunofluorescence PDGF-B Antibody
Western Blot PDGF-B Antibody
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
PDGF R beta
Antibody [Unconjugate
AF1042
Immunohistochemistry PDGF R beta Antibody [Unconjugated]
Western Blot PDGF R beta Antibody [Unconjugated]
Immunohistochemistry PDGF R beta Antibody [Unconjugated]
(110 Publications)
Species: Mu
Applications: Dual ISH-IHC, IHC, WB
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(555 Publications)
Species: Hu
Applications: BA
c-Myc Antibody
(9E10) - BSA Free
NB600-302
Flow Cytometry c-Myc Antibody (9E10) - BSA Free
Flow Cytometry c-Myc Antibody (9E10) - BSA Free
Western Blot c-Myc Antibody (9E10) - BSA Free
(4 Reviews)
(46 Publications)
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
c-Fos Antibody
(2H2)
NBP2-50037
Western Blot c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
(1 Review)
(20 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(670 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
BLOC1S2 Antibody -
BSA Free
NBP3-05039
Western Blot BLOC1S2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, WB
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(852 Publications)
Species: Hu
Applications: BA
ERMAP Antibody
[Unconjugated]
AF4928
Flow Cytometry ERMAP Antibody [Unconjugated]
Species: Hu
Applications: CyTOF-ready, Flow, WB
CD34 Antibody (MEC
14.7)
NB600-1071
Flow Cytometry CD34 Antibody (MEC 14.7)
Flow Cytometry CD34 Antibody (MEC 14.7)
Immunocytochemistry/ Immunofluorescence CD34 Antibody (MEC 14.7)
(29 Publications)
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
CD117/c-kit
Antibody [Unconjugate
AF1356
Simple Western CD117/c-kit Antibody [Unconjugated]
Western Blot CD117/c-kit Antibody [Unconjugated]
Immunohistochemistry CD117/c-kit Antibody [Unconjugated]
(4 Reviews)
(116 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Simple Western, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
ErbB2/Her2
[Unconjugated]
1129-ER
SDS-PAGE ErbB2/Her2 [Unconjugated]
Bioactivity ErbB2/Her2 [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
PDGF-B Antibody
(MM0014-5F66) - Azide ...
NB110-60980
PDGF-B Antibody (MM0014-5F66) - Azide and BSA Free
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for PDGF-B Antibody (NB110-60980)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, WB.


Filter By Application
FLOW
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for PDGF-B Antibody (NB110-60980) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Equine.

Reviews using NB110-60980:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Equine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PDGF-B NB110-60980
Enlarge 		4
reviewed by:
Verified Customer
 IHC-P Equine 11/10/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedUterus tissue
SpeciesEquine
LotMM0014-5F66

Comments

CommentsIHC (P) on equine endometrium using the antibody at 1:200 dilution.
Protein is expressed primarily in the luminal epithelium and to a lesser extent in glandular epithelium. No staining on neg. control (omission of the prim AB and replacement with mouse normal serum). Heat-induced antigen retrieval with EDTA based solution for 10 min.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PDGF-B Antibody (NB110-60980). (Showing 1 - 1 of 1 FAQs).

  1. Does Novus Bio own the cell line for Mab MM0014-5F66 (Cat# NB110-60980)?
    • Unfortunately Novus does not own the cell line for this monoclonal.

Secondary Antibodies

 

Isotype Controls

Additional PDGF-B Products

Research Areas for PDGF-B Antibody (NB110-60980)

Find related products by research area.

Blogs on PDGF-B

There are no specific blogs for PDGF-B, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
Immunohistochemistry-Paraffin PDGF-B NB110-60980
Enlarge
 Verified Customer
11/10/2017
Application: IHC-P
Species: Equine
read this review

Bioinformatics

Gene Symbol PDGFB
Entrez
Uniprot