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PCK2 Antibody (2C9)

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Western Blot: PCK2 Antibody (2C9) [H00005106-M05] - Analysis of PCK2 expression in A-431 (Cat # L015V1).
Western Blot: PCK2 Antibody (2C9) [H00005106-M05] - Analysis of PCK2 expression in A-431 (Cat # L015V1).
Sandwich ELISA: PCK2 Antibody (2C9) [H00005106-M05] - Detection limit for recombinant GST tagged PCK2 is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PCK2 Antibody (2C9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PCK2 (AAH01454, 1 a.a. ~ 640 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM
Specificity
PCK2 - phosphoenolpyruvate carboxykinase 2 (mitochondrial) (2C9)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PCK2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PCK2 Antibody (2C9)

  • EC 4.1.1.32
  • PE
  • PEPCK
  • PEPCK2PEP carboxykinase
  • PEPCK-M
  • phosphoenolpyruvate carboxykinase [GTP], mitochondrial
  • phosphoenolpyruvate carboxykinase 2 (mitochondrial)
  • Phosphoenolpyruvate carboxylase
  • phosphopyruvate carboxylase

Background

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00005106-M05
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Bioinformatics

Gene Symbol PCK2
Entrez
Uniprot