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Pax6 Antibody

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Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human endometrium shows no positivity as expected.
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human pancreas shows strong nuclear positivity in islet cells.
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in granular layer
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human stomach shows strong nuclear positivity in a subset glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Pax6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VSSFTSGSMLGRTDTALTNTYSALPPMPSFTMANNLPMQPPVPSQTSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYW
Marker
Neural Marker
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF fixation permeabilization: PFA/Triton X-100
Control Peptide
Pax6 Protein (NBP1-89100PEP)
Publications
Read Publications using
NBP1-89100 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pax6 Antibody

  • keratitis)
  • MGC17209
  • Oculorhombin
  • paired box 6
  • paired box protein Pax-6
  • Pax6

Background

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Pax6 Antibody
NBP1-89100
Pax6 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC

Publications for Pax6 Antibody (NBP1-89100)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Pax6 Antibody (NBP1-89100). (Showing 1 - 1 of 1 FAQs).

  1. What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
    • By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Pax6 Antibody (NBP1-89100)

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Blogs on Pax6.

Check out the latest blog posts on Pax6.
Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol PAX6
Entrez
Uniprot