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Pax2 Antibody (CL9469)

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Analysis in human kidney and prostate tissues using NBP2-88934 antibody. Corresponding PAX2 RNA-seq data are presented ...read more
Western Blot: Pax2 Antibody (CL9469) [NBP2-88934] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of EFO-21 cells using the Anti-PAX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and ...read more
Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human endometrium shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human kidney shows strong nuclear positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human renal cancer shows moderate nuclear positivity in tumor cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL9469
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Pax2 Antibody (CL9469) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids: ERPSYPDVFQASEHIKSEQ
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PAX2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Pax2 Antibody (CL9469)

  • paired box 2
  • paired box gene 2
  • paired box homeotic gene 2
  • paired box protein Pax-2
  • Pax2

Background

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax2 Antibody (NBP2-88934) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pax2 Antibody (NBP2-88934) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAX2