![Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0001.jpg "Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Unpurified |
| Immunogen | Residues 43-59 [QRQESKKMPVAGGKANK] of the 62 kDa human PARP-2 protein. |
| Localization | Nucleus |
| Specificity | N-terminus of human PARP 2 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PARP2 |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody can be used for western blot, where the predicted molecular weight is 66 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 66 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Purity | Whole Antiserum |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PARP2 Antibody (NB100-185)Find related products by research area.
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PARP Antibody Assays aid both Apoptosis and Cancer Research The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play... Read full blog post. |
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![Western Blot: PARP2 Antibody [NB100-185] - Western blot of analysis of PARP2 in 3T3 cell lysate using NB100-185 at 1:250 dilution.](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0002.jpg "Western Blot: PARP2 Antibody [NB100-185] - Western blot of analysis of PARP2 in 3T3 cell lysate using NB100-185 at 1:250 dilution.")
![Western Blot: PARP2 Antibody [NB100-185] - Activation of signaling pathways by dandelion root extract. Western blots of proteins involved in programmed cell death and cell survival and inflammation. The protein levels, including ULK1 (NBP2-24738), Bcl-2 related protein A1 (NBP1-76715), and PARP2 (NB100-185) corresponded to the mRNA levels obtained during the gene expression analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27564258/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb100-185_rabbit-polyclonal-parp2-antibody-unpurified-25520231539510.jpg "Western Blot: PARP2 Antibody [NB100-185] - Activation of signaling pathways by dandelion root extract. Western blots of proteins involved in programmed cell death and cell survival and inflammation. The protein levels, including ULK1 (NBP2-24738), Bcl-2 related protein A1 (NBP1-76715), and PARP2 (NB100-185) corresponded to the mRNA levels obtained during the gene expression analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27564258/) licensed under a CC-BY license.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")