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Pappalysin-1/PAPP-A Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody [NBP1-90087] - Staining in human placenta and skeletal muscle tissues . Corresponding PAPPA RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody [NBP1-90087] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody [NBP1-90087] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody [NBP1-90087] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody [NBP1-90087] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Pappalysin-1/PAPP-A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SCLDHNSESIILPMNVTVRDIPHWLNPTRVERVVCTAGLKWYPHPALIHCVKGCEPFMGDNYCDAINNRAFCNYDGGDCCTSTVKTKKVTPFPMSCDLQGDCACRDPQAQEHSRKDL
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAPPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Pappalysin-1/PAPP-A Protein (NBP1-90087PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pappalysin-1/PAPP-A Antibody

  • ASBABP2
  • DIPLA1
  • EC 3.4.24.79
  • IGFBP-4ase
  • IGFBP-4aseaspecific BCL2 ARE-binding protein 2
  • IGF-dependent IGFBP-4 protease
  • insulin-like growth factor-dependent IGF binding protein-4 protease
  • Insulin-like growth factor-dependent IGF-binding protein 4 protease
  • PAPA
  • PAPPA
  • PAPPA1
  • PAPP-A1
  • PAPP-Adifferentially placenta 1 expressed protein
  • Pappalysin1
  • Pappalysin-1
  • pregnacy-associated plasma protein A
  • Pregnancy-associated plasma protein A
  • pregnancy-associated plasma protein A, pappalysin 1

Background

PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Pappalysin-1/PAPP-A Antibody (NBP1-90087). (Showing 1 - 1 of 1 FAQ).

  1. I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).
    • Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAPPA