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p97/VCP Antibody (3P8T3)

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Western Blot: p97/VCP Antibody (3P8T3) [NBP3-15478] - Western blot analysis of extracts of various cell lines, using p97/VCP Rabbit mAb (NBP3-15478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ...read more
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] - Immunofluorescence analysis of U-2 OS cells using p97/VCP Rabbit mAb (NBP3-15478) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] - Immunofluorescence analysis of C6 cells using p97/VCP Rabbit mAb (NBP3-15478) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] - Immunofluorescence analysis of NIH-3T3 cells using p97/VCP Rabbit mAb (NBP3-15478) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
3P8T3
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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p97/VCP Antibody (3P8T3) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p97/VCP (P55072). MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVI
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
VCP
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for p97/VCP Antibody (3P8T3)

  • 15S Mg(2+)-ATPase p97 subunit
  • IBMPFD
  • IBMPFDMGC8560
  • MGC131997
  • p97
  • TER ATPase
  • TERA
  • transitional endoplasmic reticulum ATPase
  • valosin containing protein
  • Valosin-containing proteinMGC148092
  • VCP
  • yeast Cdc48p homolog

Background

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p97/VCP Antibody (NBP3-15478) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VCP