Novus Biologicals products are now on bio-techne.com

p70 S6 Kinase beta/S6K2 Antibody

Images

 
Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells ...read more
Western Blot: p70 S6 Kinase beta/S6K2 Antibody [NB100-1545] - Whole cell lysate (5 and 15 ug) from HeLa cells. Antibody used at 0.04 ug/ml for WB.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

p70 S6 Kinase beta/S6K2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 430 and the C-terminus (residue 482) of human Ribosomal Protein S6 Kinase, 70kda, polypeptide 2 using the numbering given in entry NP_003943.2 (GeneID 6199).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6KB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 -6 ug/mg of lysate
  • Western Blot 1:2000-1:10000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
p70 S6 Kinase beta/S6K2 Overexpression Lysate
Publications
Read Publications using NB100-1545.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p70 S6 Kinase beta/S6K2 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • KLS
  • p70 ribosomal S6 kinase beta
  • p70 S6 Kinase beta
  • p70 S6KB
  • p70 S6K-beta
  • p70(S6K)-beta
  • p70-beta
  • P70-beta-1
  • P70-beta-2
  • p70-S6K 2
  • P70S6K2
  • p70S6Kb
  • ribosomal protein S6 kinase beta-2
  • ribosomal protein S6 kinase, 70kD, polypeptide 2,70 kDa ribosomal protein S6 kinase 2
  • ribosomal protein S6 kinase, 70kDa, polypeptide 2
  • RPS6KB2
  • S6K2
  • S6K-beta
  • S6K-beta2
  • S6K-beta-2
  • serine/threonine-protein kinase 14 beta
  • Serine/threonine-protein kinase 14B
  • SRK
  • STK14B
  • STK14BS6 kinase-related kinase

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1595
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP2-20362
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-87581
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-44520
Species: Ca(-), Hu, Rt(-)
Applications: Flow-IC, Flow, ICC/IF, IF, IHC, IHC-P, MI, WB
NBP1-87799
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP2-46404
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
MAB79671
Species: Hu, Mu, Rt
Applications: ICC, Simple Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
MAB6210
Species: Hu
Applications: Simple Western, WB
233-FB
Species: Hu
Applications: BA
NB100-1545
Species: Hu, Mu
Applications: WB, IP

Publications for p70 S6 Kinase beta/S6K2 Antibody (NB100-1545)(2)

Reviews for p70 S6 Kinase beta/S6K2 Antibody (NB100-1545) (0)

There are no reviews for p70 S6 Kinase beta/S6K2 Antibody (NB100-1545). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for p70 S6 Kinase beta/S6K2 Antibody (NB100-1545). (Showing 1 - 1 of 1 FAQs).

  1. I wondered if your S6k2 antibody cross reacted with S6K1 or is it specific to S6K2 NB100-1545
    • There is no mention of specifically crossreacting with any other protein but the epitope recognized by this antibody is found between aa430 and 482 of the human Ribosomal protein S6 kinase. Our lab said that there is some similarity between S6K1 and S6K2 in the sequences of the immunizing peptide. This antibody preferentially binds to S6K2 (as seen in data sheet) and it is possible under certain conditions that it would cross-react with S6K1.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional p70 S6 Kinase beta/S6K2 Products

Research Areas for p70 S6 Kinase beta/S6K2 Antibody (NB100-1545)

Find related products by research area.

Blogs on p70 S6 Kinase beta/S6K2

There are no specific blogs for p70 S6 Kinase beta/S6K2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our p70 S6 Kinase beta/S6K2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol RPS6KB2
Entrez
Uniprot