![Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human prostate cancer stained with p40/deltaNp63 antibody (P40/7302R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/p40-deltaNp63-Antibody-P40-7302R-Immunohistochemistry-Paraffin-NBP3-13806-img0002.jpg "Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human prostate cancer stained with p40/deltaNp63 antibody (P40/7302R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | P40/7302R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14057) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment (around aa1-200) of human p40/deltaNp63 protein (exact sequence is proprietary) (Uniprot: Q9H3D4 ) |
| Localization | Nucleus |
| Marker | Squamous, Basal & Myoepithelial Cell Marker |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TP63 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes |
| Theoretical MW | 63 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human prostate cancer stained with p40/deltaNp63 antibody (P40/7302R). Inset: PBS instead of primary antibody, secondary antibody negative control.](http://images.novusbio.com/fullsize/p40-deltaNp63-Antibody-P40-7302R-Immunohistochemistry-Paraffin-NBP3-13806-img0001.jpg "Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human prostate cancer stained with p40/deltaNp63 antibody (P40/7302R). Inset: PBS instead of primary antibody, secondary antibody negative control.")
![Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human ovarian cancer stained with p40/deltaNp63 antibody (P40/7302R). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/p40-deltaNp63-Antibody-P40-7302R-Immunohistochemistry-Paraffin-NBP3-13806-img0003.jpg "Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human ovarian cancer stained with p40/deltaNp63 antibody (P40/7302R). Inset: PBS instead of primary antibody; secondary only negative control.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
