Human OX40/TNFRSF4 ELISA Kit (Colorimetric) Summary
Description |
The human sCD134/OX40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD134/OX40. |
Specificity |
The assay detects both natural and recombinant human OX40/TNFRSF4. The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human OX40/TNFRSF4 positive serum. There was no crossreactivity detected. |
Standard Curve Range |
78 - 5,000 pg/mL |
Sensitivity |
1.8 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 12.0% |
Intra-Assay |
CV% < 8.0% |
Spike Recovery |
88% |
Sample Volume |
25 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
TNFRSF4 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD134/OX40
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human sCD134/OX40 monoclonal antibody
- Blue-Dye
- Green-Dye
- Human sCD134/OX40 Standard lyophilized, 10 ng/ml upon reconstitution
- Red-Dye
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
|
Alternate Names for Human OX40/TNFRSF4 ELISA Kit (Colorimetric)
Background
The human sCD134/OX40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD134/OX40.
OX40 (CD134) is a member of the tumor necrosis (TNF) receptor superfamily and known to be an important costimulatory molecule expressed on activated T-cells. Interaction of OX40 with its ligand, OX40L, is thought to be important in T cell activation through T cell/antigen-presenting cell (APC) interaction. Ligation of OX40 induces clonal expansion and survival of CD4 cells during primary responses, and results in the accumulation of greater numbers of memory cells with time.
A soluble isoform of OX40 has been described. Measurement of this molecule may have diagnostic value in polymyositis and granulomatous myopathy, in T cell lymphoma and lymphomatoid papulosis, in proliferative lupus nephritis, in rheumatoid arthritis, in HIV infection, in viral infections of the lung, in the regulation of graft-versus-host disease,in myocarditis and dilated cardiomyopathy.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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