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Human OX40/TNFRSF4 ELISA Kit (Colorimetric)

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ELISA: Human OX40/TNFRSF4 ELISA Kit (Colorimetric) [NBP2-22043]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
78 - 5,000 pg/mL
Sensitivity
1.8 pg/mL

Order Details

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Human OX40/TNFRSF4 ELISA Kit (Colorimetric) Summary

Description
The human sCD134/OX40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD134/OX40.
Specificity
The assay detects both natural and recombinant human OX40/TNFRSF4. The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human OX40/TNFRSF4 positive serum. There was no crossreactivity detected.
Standard Curve Range
78 - 5,000 pg/mL
Sensitivity
1.8 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12.0%
Intra-Assay
CV% < 8.0%
Spike Recovery
88%
Sample Volume
25 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFRSF4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD134/OX40
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human sCD134/OX40 monoclonal antibody
  5. Blue-Dye
  6. Green-Dye
  7. Human sCD134/OX40 Standard lyophilized, 10 ng/ml upon reconstitution
  8. Red-Dye
  9. Sample Diluent
  10. Stop Solution (1M Phosphoric acid)
  11. Streptavidin-HRP
  12. Substrate Solution (tetramethyl-benzidine)
  13. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human OX40/TNFRSF4 ELISA Kit (Colorimetric)

  • ACT-135
  • ACT35 antigen
  • ACT35ATC35 antigen
  • CD134 antigen
  • CD134
  • Ly-70
  • OX40 cell surface antigen
  • OX40 homologue
  • OX40
  • OX40L receptor
  • OX40lymphoid activation antigene ACT35
  • TAX transcriptionally-activated glycoprotein 1 receptor
  • tax-transcriptionally activated glycoprotein 1 receptor
  • TNFRSF4
  • tumor necrosis factor receptor superfamily member 4
  • tumor necrosis factor receptor superfamily, member 4
  • Txgp1
  • TXGP1L
  • TXGP1LOX40 antigen

Background

The human sCD134/OX40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD134/OX40.

OX40 (CD134) is a member of the tumor necrosis (TNF) receptor superfamily and known to be an important costimulatory molecule expressed on activated T-cells. Interaction of OX40 with its ligand, OX40L, is thought to be important in T cell activation through T cell/antigen-presenting cell (APC) interaction. Ligation of OX40 induces clonal expansion and survival of CD4 cells during primary responses, and results in the accumulation of greater numbers of memory cells with time.
A soluble isoform of OX40 has been described. Measurement of this molecule may have diagnostic value in polymyositis and granulomatous myopathy, in T cell lymphoma and lymphomatoid papulosis, in proliferative lupus nephritis, in rheumatoid arthritis, in HIV infection, in viral infections of the lung, in the regulation of graft-versus-host disease,in myocarditis and dilated cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF4