Home » Osteopontin/OPN » Osteopontin/OPN Proteins » Osteopontin/OPN Recombinant Protein Antigen
Novus Biologicals products are now on bio-techne.com

Osteopontin/OPN Recombinant Protein Antigen

 Submit a Review

Images

 
There are currently no images for Osteopontin/OPN Protein (NBP1-89952PEP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Osteopontin/OPN Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human SPP1.

Source: E. coli

Amino Acid Sequence: SQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDAT

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
SPP1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-89952.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Osteopontin/OPN Recombinant Protein Antigen

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Osteocalcin
Antibody (190125)
MAB1419
Intracellular Staining by Flow Cytometry Osteocalcin Antibody (190125)
Intracellular Staining by Flow Cytometry Osteocalcin Antibody (190125)
Immunocytochemistry Osteocalcin Antibody (190125)
(3 Reviews)
(56 Publications)
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
IBSP/Sialoprotein
II [Unconjugated]
4014-SP
Bioactivity IBSP/Sialoprotein II [Unconjugated]
(5 Publications)
Species: Hu
Applications: BA
SPARC Antibody
[Unconjugated]
AF942
Western Blot SPARC Antibody [Unconjugated]
Immunohistochemistry SPARC Antibody [Unconjugated]
Immunocytochemistry SPARC Antibody [Unconjugated]
(38 Publications)
Species: Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow, WB
RUNX2/CBFA1
Antibody (232902) [Un ...
MAB2006
Immunocytochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]
(1 Review)
(28 Publications)
Species: Hu
Applications: ICC
SLPI [HRP]
DPI00
N/A SLPI [HRP]
N/A SLPI [HRP]
(37 Publications)
Species: Hu
Applications: ELISA
Alkaline
Phosphatase/ALPP Anti ...
NB110-3638
Immunocytochemistry/ Immunofluorescence Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
Immunohistochemistry-Paraffin Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
Western Blot Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
(1 Review)
(15 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
CCL27/CTACK [HRP]
DCC270
N/A CCL27/CTACK [HRP]
N/A CCL27/CTACK [HRP]
(10 Publications)
Species: Hu
Applications: ELISA
NAT10 Antibody
NBP2-32263
Immunoprecipitation NAT10 Antibody
Western Blot NAT10 Antibody
Immunoprecipitation NAT10 Antibody
Species: Hu, Mu
Applications: IHC, IP, WB
ATRNL1 Antibody -
Azide and BSA Free
NBP2-92749
Western Blot ATRNL1 Antibody - Azide and BSA Free
Species: Hu, Mu
Applications: WB
ASRGL1 Antibody
NBP1-89133
Immunocytochemistry/ Immunofluorescence ASRGL1 Antibody
Immunohistochemistry-Paraffin ASRGL1 Antibody
Immunohistochemistry-Paraffin ASRGL1 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
PDLIM3 Antibody
(HL2075) - Azide and ...
NBP3-25358
Immunohistochemistry-Paraffin PDLIM3 Antibody (HL2075) - Azide and BSA Free
Immunohistochemistry-Paraffin PDLIM3 Antibody (HL2075) - Azide and BSA Free
Western Blot PDLIM3 Antibody (HL2075) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB

Osteoprotegerin/TNFRSF
DY805
N/A Osteoprotegerin/TNFRSF11B [Biotin]
(89 Publications)
Species: Hu
Applications: ELISA
BMP-2
[Unconjugated]
355-BM
Bioactivity BMP-2 [Unconjugated]
(190 Publications)
Species: Hu, Mu, Rt
Applications: BA
CD44 Antibody
(2C5) [Unconjugated] ...
BBA10
Simple Western CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
Western Blot CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
Immunohistochemistry CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
(5 Reviews)
(49 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
Fibronectin
Antibody - BSA Free
NBP1-91258
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
(11 Reviews)
(56 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(817 Publications)
Species: Hu
Applications: BA
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(684 Publications)
Species: Hu
Applications: ELISA
DSPP Antibody -
Azide and BSA Free
NBP2-92546
Western Blot DSPP Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence DSPP Antibody - Azide and BSA Free
(3 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(663 Publications)
Species: Mu
Applications: ELISA
Osteopontin/OPN
Recombinant Protein A ...
NBP1-89952PEP
Osteopontin/OPN Recombinant Protein Antigen
Species: Hu
Applications: AC

Publications for Osteopontin/OPN Protein (NBP1-89952PEP) (0)

There are no publications for Osteopontin/OPN Protein (NBP1-89952PEP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Osteopontin/OPN Protein (NBP1-89952PEP) (0)

There are no reviews for Osteopontin/OPN Protein (NBP1-89952PEP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Osteopontin/OPN Protein (NBP1-89952PEP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Osteopontin/OPN Products

Research Areas for Osteopontin/OPN Protein (NBP1-89952PEP)

Find related products by research area.

Blogs on Osteopontin/OPN.

Check out the latest blog posts on Osteopontin/OPN.
Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Osteopontin/OPN Antibody [Unconjugated]
AF808

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)

As an example, if the molecular weight of a compound is 197.13 g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = 19.713 (value determined by this calculator).

=
x
x
g/mol

Review this Product

Be the first to review our Osteopontin/OPN Recombinant Protein Antigen and receive a gift card or discount.


Bioinformatics

Gene Symbol SPP1