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Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

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ELISA: Monkey Osteopontin/OPN ELISA Kit (Colorimetric) [NBP2-68076] - Samples were spiked with high concentrations of Monkey Osteopontin/OPN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Monkey Osteopontin/OPN ELISA Kit (Colorimetric) [NBP2-68076] - Samples were spiked with high concentrations of Monkey Osteopontin/OPN and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Monkey Osteopontin/OPN ELISA Kit (Colorimetric) [NBP2-68076] - Standard Curve Reference

Product Details

Summary
Reactivity PmSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Monkey Osteopontin/OPN ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Monkey OPN in samples. No significant cross-reactivity or interference between Monkey OPN and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.33%
Intra-Assay
CV% < 4.62%
Spike Recovery
83-110%
Sample Volume
100uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SPP1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Monkey Osteopontin/OPN ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol SPP1