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Olig1/2/3 Antibody (257224) [Unconjugated]

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Olig 1, 2, 3 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Mouse Anti-Human Olig 1, 2, 3 Monoclonal Antibody (Catalog # MAB2230) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons in developing brain. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Olig 1, 2, 3 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Mouse Anti-Human Olig 1, 2, 3 Monoclonal Antibody (Catalog # MAB2230) at 15 µg/mL for 1 hour ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ICC/IF
Clone
257224
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Phycoerythrin
IC2230P
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Catalog# & Formulation Size Price
Carrier Free
IC2230P
Carrier Free
MAB2230-SP
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Olig1/2/3 Antibody (257224) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Olig1
aa 1-272
Specificity
Detects human Olig 1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross‑reactivity with recombinant human (rh) Olig2 and 50 - 100% cross‑reactivity with rhOlig3.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB2230 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig1/2/3 Antibody (257224) [Unconjugated]

  • bHLHb6
  • bHLHe21
  • Olig 1, 2, 3

Background

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in neural tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB2230
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Publications for Olig1/2/3 Antibody (MAB2230)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, IHC-P.


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