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Olig1/2/3 Antibody (257224) [Phycoerythrin]

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A172 human glioblastoma cell line was stained with Mouse Anti-Human Olig1, 2, 3 PE-conjugated Monoclonal Antibody (Catalog # IC2230P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
A172 human glioblastoma cell line was stained with Mouse Anti-Human Olig1, 2, 3 PE-conjugated Monoclonal Antibody (Catalog # IC2230P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
257224
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
MAB2230
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2230
Carrier Free
MAB2230
Carrier Free
MAB2230-SP
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Olig1/2/3 Antibody (257224) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human Olig1
aa 1-272
Specificity
Detects human Olig1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) Olig2 and 50-100% cross-reactivity with rhOlig3.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig1/2/3 Antibody (257224) [Phycoerythrin]

  • bHLHb6
  • bHLHe21
  • Olig 1, 2, 3

Background

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adults, expression of Olig3 is primarily detected in neural tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Olig1/2/3 Antibody
(257224) [Phycoerythr ...
IC2230P
Olig1/2/3 Antibody (257224) [Phycoerythrin]
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