![SDS-Page: Recombinant Human OGG1 His Protein [NBP1-45318] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-OGG1-His-Protein-SDS-Page-NBP1-45318-img0001.jpg "SDS-Page: Recombinant Human OGG1 His Protein [NBP1-45318] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
| Reactivity | HuSpecies Glossary |
| Applications | PAGE |
| Concentration | 0.5 mg/ml |
| Description | A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-345 of Human OGG1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH TGSMPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLT QTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVG TKVADCICLM ALDKPQAVPV DVHMWHIAQRDYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRR KGSKGPEG |
| Details of Functionality | Glycerol inhibits binding affinity to Ni-NTA beads in immunoprecipitation. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | OGG1 |
| Purity | >90%, by SDS-PAGE |
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| Application Notes | Use in In vitro assay reported in scientific literature (PMID 24489103). |
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| Theoretical MW | 41.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 40% glycerol |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Purity | >90%, by SDS-PAGE |
Research Areas for OGG1 Recombinant Protein (NBP1-45318)Find related products by research area.
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You down with Ogg? Yeah, you know me 8-hydroxyguanine is a form of oxidative DNA damage where free radicals cause G:C to T:A transversions within the backbone. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine mutations. One of these enzymes,... Read full blog post. |
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| Gene Symbol | OGG1 |
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![Flow Cytometry: OGG1 Antibody [NB100-106] - An intracellular stain was performed on Jurkat Cells with NB100-106 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](https://images.novusbio.com/images/OGG1-Antibody-Flow-Cytometry-NB100-106-img0029.jpg "Flow Cytometry: OGG1 Antibody [NB100-106] - An intracellular stain was performed on Jurkat Cells with NB100-106 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.")