![Western Blot: Ogg1 Overexpression Lysate (Adult Normal) [NBP2-09881] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ogg1.](http://images.novusbio.com/fullsize/Ogg1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09881-img0001.jpg "Western Blot: Ogg1 Overexpression Lysate (Adult Normal) [NBP2-09881] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ogg1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | OGG1 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC221725 Accession#: NM_016821 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | OGG1 |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 47.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
Research Areas for OGG1 Lysate (NBP2-09881)Find related products by research area.
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You down with Ogg? Yeah, you know me 8-hydroxyguanine is a form of oxidative DNA damage where free radicals cause G:C to T:A transversions within the backbone. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine mutations. One of these enzymes,... Read full blog post. |
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| Gene Symbol | OGG1 |
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![Flow Cytometry: OGG1 Antibody [NB100-106] - An intracellular stain was performed on Jurkat Cells with NB100-106 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](https://images.novusbio.com/images/OGG1-Antibody-Flow-Cytometry-NB100-106-img0029.jpg "Flow Cytometry: OGG1 Antibody [NB100-106] - An intracellular stain was performed on Jurkat Cells with NB100-106 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.")