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Oct-3/4 Antibodies

We offer Oct-3/4 Antibodies for use in common research applications: Chromatin Immunoprecipitation, Flow (Intracellular), Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot. Each Oct-3/4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Oct-3/4 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the Oct-3/4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Oct-3/4 polyclonal antibodies and browse our Oct-3/4 monoclonal antibody catalog.

Alternate Names for Oct-3/4 Antibodies

anti-Oct-3/4 antibody, anti-POU5F1 antibody

3 Results for "oct-3-4" in Primary Antibodies

AF1759

Oct-3/4 Antibody [Unconjugated]

(77 Publications)

100 μg

AF1759

Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Oct-3/4 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Alkaline phosphatase (ALPL) and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct‑3/4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.

Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct‑3/4 at approximately 50 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockdown OGT inhibits proliferation and tumorsphere formation of hepatoma cell through reducing eIF4E expression. A, Huh7 and PLC/PRF/5 cells were infected with control shRNA, OGT shRNA2 alone, or with wild‐type eIF4E lentivirus. The cell lysates were harvested for western blotting analysis using indicated antibodies. beta ‐actin expression was served as a loading control. B, Cell proliferation of Huh7 and PLC/PRF/5 cells infected with lentiviruses as in panel (A) were measured with CCK8 assay. (C‐H) Huh7 and PLC/PRF/5 cells infected with lentiviruses as in panel (A) were seeded into 96‐well plates. After 12 d, tumorsphere were counted and quantified. Representative images of sphere (scale bars, 100 μm) were shown (C, F). The diameter of sphere (D, G) and number of sphere (E, H) were count. Data represent mean ± SD of at least three independent experiments. The two‐tailed Student's t tests were used. **P < 0.01. I, Huh7 cells expressing either OGT shRNA2 alone or with wild‐type eIF4E lentivirus were incubated with PE‐labelled anti‐AC133 antibody. The percentages of CD133+ cells in graphs were analysed by flow cytometry. Black line, control IgG staining; red line, CD133 staining. J, Cell lysates were examined by western blotting with indicated antibodies. The right panel showcases relative protein amounts of different groups. Error bars represent ±SD of triplicate experiments. **P < 0.01; n.s, no significance. K, Huh7 cells were collected and subjected to immunoprecipitation with antibody against eIF4E or normal mouse IgG. Total RNAs were purified from immunocomplexes and subjected to RT‐PCR to measure Sox2, OCT4, and KLF4 mRNAs associated with eIF4E Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30677218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neural induction of hPSCs. (a) Both hPSC culture and neural differentiation were performed on LN521 substrate. Cortical neurons were induced with dual SMAD inhibition, expanded in the presence of FGF2, and matured with support by a selection of neurotrophic factors. Neural progenitor cells (NPCs) could be cryopreserved at day 21 and plated at day 32 for final experiments, including microelectrode array (MEA) measurements. (b) Three different hPSC lines (one hESC line, 08/023 and two hiPSC lines, 10212.EURCCs and IMR90-4) were characterized for their efficiency in producing neuroectodermal cells in response to 12-day neural induction by dual SMAD inhibition. Cells were stained for pluripotency marker Oct4 at the pluripotent stage and after 12 days of neural induction. The presence of early neuroectodermal markers was evaluated with Sox2, FoxG1 and Pax6 staining. (c) After 21 days of differentiation, the culture contained vimentin- and Pax6-positive NPCs that could be cryopreserved. Additionally, the first Tbr2- and MAP2-positive neurons were detected at this time point. (d) The percentages of Pax6-positive cells were quantified at four time points of differentiation (mean ± s.e.m., n = 5–14, data derived from 1–3 independent differentiations). Statistical analysis was performed with the Mann-Whitney U test to compare differences between day 12 and day 46 within each hPSC line, and significant p-values are presented in the image. The scale bar is 50 µm in all images. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31748598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1759

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1759-SP

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MAB1759

Oct-3/4 Antibody (240408) [Unconjugated]

(47 Publications)

100 μg

MAB1759

Western blot shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (Catalog # MAB1759) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Oct-3/4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Oct‑3/4 was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using Rhodamine Red-coupled anti-rat IgG (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Pertussis toxin does not affect the maintenance of pluripotency pluripotent stem cell colonies or the ability to form all three embryonic germ layers.(A) Pertussis toxin does not affect the maintenance of pluripotency of hES or hiPS cells as assessed by the expression of the pluripotency markers Nanog, Oct4, Sox2, TDGF1, and ZFP42 by quantitative real-time PCR. (B) Immunocytochemistry of TRA-1-81 (green) and Oct-3/4 (red) also did not reveal differences between hES or hiPS cells treated with pertussis toxin and control treatments in pluripotent stem cell colonies. Nuclei were stained with Hoechst (blue). Scale bar, 200 µm. (C) hES or iPS cells treated with pertussis toxin formed embryoid bodies and differentiated into cell types of the all three embryonic germ layers as assessed by immunocytochemistry for alpha -fetoprotein (a marker of endoderm), alpha -smooth muscle actin (mesoderm) and beta III-tubulin (ectoderm). Scale bar, 100 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19936228), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of DFX on spherogenicity of human cancer cell lines and treatment with Nanog siRNA in vitro. (A) After treatment with 0.2% DMSO or 50 μM DFX, a single suspension of HSC-2 cells or OE33 cells was used for the sphere formation assay in a 96-well ultra-low attachment plate. DFX suppressed the spherogenicity of HSC-2 cells and OE33 cells. (B) A single suspension of HSC-2 cells or OE33 cells as described above was used for the spheroid colony assay in a 24-well ultra-low attachment plate. The number of spheres over 50 μm in diameter was counted. The experiments were performed in triplicate, and means ± S.E.M. of each group are shown. DFX significantly suppressed the number of spheres. * p < 0.05. (C) HSC-2 cells were transfected with control or si-Nanog for 48 h, and the expression of stemness markers (Nanog, Sox2, Oct3/4, Klf4, c-Myc) was determined with western blot analysis. beta -actin was used as a loading control. siRNA suppressed the expression of Nanog, Oct3/4, and Klf4. (D) HSC-2 cells were transfected with control or si-Nanog for 48 h, and the sphere formation assay was performed. No differences were found in spherogenicity between the control and si-Nanog cultures. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30717462), licensed under a CC-BY license. Not internally tested by R&D Systems.

Undifferentiated H7 hESC colonies (on passage 15) 3 days after passaging on hFF feeder cells (a) and after 55 passages on MEF feeder cells (b). H7 cells passaged 11 times on hFF feeder cells expressed Nanog (c and d), Oct4 (e and f), SSEA4 (g and h), and TRA-1-60 (i and j). H7 cells on passage 45 on MEF feeder cells expressed Nanog (k and l), Oct4 (m and n), and SSEA4 (o and p). Scale bars 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22315618), licensed under a CC-BY license. Not internally tested by R&D Systems.

Undifferentiated H7 hESC colonies (on passage 15) 3 days after passaging on hFF feeder cells (a) and after 55 passages on MEF feeder cells (b). H7 cells passaged 11 times on hFF feeder cells expressed Nanog (c and d), Oct4 (e and f), SSEA4 (g and h), and TRA-1-60 (i and j). H7 cells on passage 45 on MEF feeder cells expressed Nanog (k and l), Oct4 (m and n), and SSEA4 (o and p). Scale bars 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22315618), licensed under a CC-BY license. Not internally tested by R&D Systems.

The effect of DFX against stemness of miPS cells in vitro and cytotoxicity analysis. (A) miPS cells were treated with the indicated dose of DFX (0, 1, 10, 50, 100 μM) and subjected to western blot analysis with antibodies to stemness markers (Nanog, Sox2, Oct3/4, Klf4, c-Myc) or beta -actin (loading control). Stemness markers were suppressed by DFX at concentrations over 50 μM. (B) miPS cells treated with 50 μM DFX were cultured in suspension for 72 h. DFX treatment of miPS cells suppressed spherogenesis and GFP expression, which indicates suppression of Nanog. (C) Micrographs of the fluorescence-based Live/Dead assay showing live and dead miPS cells following treatment with 0.2% DMSO (control) or 50 μM DFX (magnification ×40). The morphology of miPS cells after treatment with DFX changed from round to spindle shaped. Almost all cells were stained green, which indicates live cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30717462), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1759U-100UG
Alexa Fluor 405	IC1759V-100UG
Alexa Fluor 488	IC1759G-100UG
Alexa Fluor 532	FAB1759X
Alexa Fluor 594	IC1759T-100UG
Alexa Fluor 647	IC1759R-100UG
Alexa Fluor 700	IC1759N-100UG
Alexa Fluor 750	IC1759S-100UG
Allophycocyanin	IC1759A-100
Allophycocyanin	IC1759A-025
Biotin	FAB1759B
CoraFluor 1	FAB1759CL1
DyLight 350	FAB1759D
DyLight 405	FAB1759E
DyLight 488	FAB1759K
DyLight 550	FAB1759L
DyLight 594	FAB1759M
DyLight 650	FAB1759W
DyLight 680	FAB1759Y
DyLight 755	FAB1759Z
Fluorescein	IC1759F-025
Fluorescein	IC1759F-100
HRP	FAB1759H
Janelia Fluor 525	FAB1759JF525
Janelia Fluor 549	FAB1759I
Janelia Fluor 646	FAB1759J
Janelia Fluor 669	FAB1759JF669
Phycoerythrin	IC1759P-025
Phycoerythrin	IC1759P-100
mFluor Violet 450 SE	FAB1759MFV450
mFluor Violet 500 SE	FAB1759MFV500
mFluor Violet 610 SE	FAB1759MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1759-SP
Carrier Free	IC1759A-100
Carrier Free	IC1759A-025
Carrier Free	IC1759F-100
Carrier Free	IC1759F-025
Carrier Free	IC1759P-100
Carrier Free	IC1759P-025
Carrier Free	IC1759V-100UG
Carrier Free	IC1759U-100UG
Carrier Free	IC1759T-100UG
Carrier Free	IC1759S-100UG
Carrier Free	IC1759R-100UG
Carrier Free	IC1759N-100UG
Carrier Free	IC1759G-100UG

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NBP2-00352

Oct-3/4 Antibody (EM92)

100 μg

NBP2-00352

Western Blot: OCT3/4 Antibody (EM92) [NBP2-00352] - F9 mouse embryonal carcinoma cell line (left) and mouse splenocyte (right) lysates were loaded at 1x10e5 cells/lane, probed with 2 ug/mL of Anti-Mouse OCT3/4 Purified and revealed with Anti-Rat IgG HRP.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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