![Western Blot: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Western-Blot-NBP2-01446-img0010.jpg "Western Blot: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI1A5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.93 mg/ml |
| Immunogen | Full length human recombinant protein of human NUCB1(NP_006175) produced in HEk293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NUCB1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 53.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.93 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-01446 | Applications | Species |
|---|---|---|
| Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01 |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nucleobindin 1.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Immunocytochemistry-Immunofluorescence-NBP2-01446-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nucleobindin 1.")
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Staining of paraffin-embedded Human endometrium tissue using anti-Nucleobindin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-01446-img0008.jpg "Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Staining of paraffin-embedded Human endometrium tissue using anti-Nucleobindin 1 mouse monoclonal antibody.")
![Flow Cytometry: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nucleobindin 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Flow-Cytometry-NBP2-01446-img0001.jpg "Flow Cytometry: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nucleobindin 1 antibody, and then analyzed by flow cytometry.")
![Western Blot: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nucleobindin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nucleobindin 1.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Western-Blot-NBP2-01446-img0002.jpg "Western Blot: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nucleobindin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nucleobindin 1.")
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Nucleobindin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-1A5-Immunohistochemistry-Paraffin-NBP2-01446-img0007.jpg "Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Nucleobindin 1 mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")