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Nrf2 Antibody (383727) [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with ...read more
Confirmation of the IPA-predicted NRF2 activation in cancer cell lines by Western blot. Western blot analysis with NRF2-specific antibody in four different cell extracts per cell line (SiHa, HeLa, C33A, and HCK1T), ...read more
Immunoblot analysis of kidney lysates.(A) Blot membranes were incubated with the antibodies against Nrf2, p53 and 8-oxoguanine glycosylase alpha (OGG1 alpha ). The mean integrated optical density is related to actin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
383727
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Nrf2 Antibody (383727) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236
Specificity
Detects human Nrf2 in Western blots. In Western blots, no cross-reactivity with mouse and rat Nrf2 and human Nrf1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NFE2L2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4
using
MAB3925 in the following application:

Publications
Read Publications using
MAB3925 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nrf2 Antibody (383727) [Unconjugated]

  • HEBP1
  • NFE2L2
  • NF-E2-related factor 2
  • NFE2-related factor 2
  • NRF2 erythroid derived 2, like 2
  • Nrf2
  • nuclear factor (erythroid-derived 2)-like 2
  • nuclear factor erythroid 2-related factor 2
  • nuclear factor erythroid-derived 2-like 2

Background

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nrf2 Antibody (MAB3925)(41)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC, Neutralization, Western Blot.


Filter By Application
ICC
(1)
Neutralization
(1)
Western Blot
(18)
All Applications
Filter By Species
Human
(7)
Mouse
(8)
Rat
(4)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using MAB3925 Applications Species
Jramne-Saleem, Y;Danilenko, M; Roles of Glutathione and AP-1 in the Enhancement of Vitamin D-Induced Differentiation by Activators of the Nrf2 Signaling Pathway in Acute Myeloid Leukemia Cells International journal of molecular sciences 2024-02-14 [PMID: 38396960] (Western Blot, Human) Western Blot Human
Joo-Hee Park, Choul Yong Park Effects of In Vitro Combination of Nitric Oxide Donors and Hypochlorite on Acanthamoeba castellanii Viability Transl Vis Sci Technol 2023-09-28 [PMID: 37768280]
Carlos Guerrero-Bobadilla, Irinea Yáñez-Sánchez, Talia Franco-Ávila, Abril B Martínez-Rizo, Alfredo Domínguez-Rosales, Bertha Adriana Alvarez-Rodríguez et al. Reduction of NrF2 as coadjuvant during the development of persistent periapical lesions Medicina Oral Patología Oral y Cirugia Bucal 2023-09-01 [PMID: 37330967]
Gloria Mas, Na Man, Yuichiro Nakata, Concepcion Martinez-Caja, Daniel L Karl, Felipe Beckedorff et al. The SWI/SNF chromatin remodeling subunit DPF2 facilitates NRF2-dependent anti-inflammatory and anti-oxidant gene expression Journal of Clinical Investigation 2023-07-03 [PMID: 37200093]
Joo-Hee Park, Kangmin Lee, Choul Yong Park Effect of Magnetic Microparticles on Cultivated Human Corneal Endothelial Cells Translational Vision Science & Technology 2023-02-09 [PMID: 36757343]
S Sasaki, T Negishi, T Tsuzuki, K Yukawa Diphenylarsinic acid induced activation of MAP kinases, transcription factors, and oxidative stress-responsive factors and hypersecretion of cytokines in cultured normal human cerebellar astrocytes Neurotoxicology, 2021-12-06;88(0):196-207. 2021-12-06 [PMID: 34883095] (Western Blot, Human) Western Blot Human
Mateja Mikulčić, Nassim Ghaffari Tabrizi-Wizsy, Eva M. Bernhart, Martin Asslaber, Christopher Trummer, Werner Windischhofer et al. 15d-PGJ2 Promotes ROS-Dependent Activation of MAPK-Induced Early Apoptosis in Osteosarcoma Cell In Vitro and in an Ex Ovo CAM Assay International Journal of Molecular Sciences 2021-10-29 [PMID: 34769194]
Walaa Mohamed Sayed Quercetin Alleviates Red Bull Energy Drink-Induced Cerebral Cortex Neurotoxicity via Modulation of Nrf2 and HO-1 Oxidative Medicine and Cellular Longevity 2021-10-31 [PMID: 34754366]
Zhao S, Chen W, Li W et al. LncRNA TUG1 attenuates ischaemia-reperfusion-induced apoptosis of renal tubular epithelial cells by sponging miR-144-3p via targeting Nrf2 Journal of cellular and molecular medicine 9/21/2021 [PMID: 34547172]
G Kaufman, D Skrtic N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM Int J Mol Sci, 2020-10-03;21(19):. 2020-10-03 [PMID: 33023018] (ICC, Human) ICC Human
Show All 41 Publications.

Reviews for Nrf2 Antibody (MAB3925) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using MAB3925:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Nrf2 MAB3925
Enlarge
3
reviewed by:
YONGCHUL JANG
WB Mouse 09/29/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain (substantia nigra)
SpeciesMouse
LotMAB3925
  5
reviewed by:
Verified Customer
WB Mouse 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19282378
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Human 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21976775
SpeciesHuman

Product General Protocols

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FAQs for Nrf2 Antibody (MAB3925) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Nrf2 Products

Blogs on Nrf2.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.


  Read full blog post.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Crosstalk Between Oxidative Stress and Autophagy
By Christina Towers, PhD. Role of Reactive Species in Cellular FunctionOxidative stress is a byproduct of an imbalance between oxidants and antioxidants present in the cell, resulting in dysfunctional redox si...  Read full blog post.

PGC-1 alpha: Roles in Mitochondrial Biogenesis and Disease
An important aspect of mitochondria maintenance includes biogenesis to replenish damaged and degraded mitochondrial structures. The regulation of mitochondrial biogenesis is very complex and numerous genes regulate and synchronize protein synthesis fr...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

YONGCHUL JANG
09/29/2017
Application: WB
Species: Mouse

 
Verified Customer
02/20/2015
Application: WB
Species: Mouse

 
Verified Customer
02/20/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol NFE2L2
Uniprot