Immunohistochemistry-Paraffin: NOXO1 Antibody [NB300-950] - IHC analysis of NOXO1 in formalin-fixed, paraffin-embedded human kidneyusing this antibody at 10 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, ...read more
WB: Preliminary experiments gave no signal but low background in Hela and human heart lysates at up to 1 ug/ml. IHC-P: Human kidney cortex shows cytoplasmic stain in epithelial cells of some PCT.
Mouse reactivity reported in scientific literature (PMID: 28824611).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified
Alternate Names for NOXO1 Antibody
MGC20258
NADPH oxidase organizer 1
Nox organizer 1
Nox-organizing protein 1
P41NOX
P41NOXA
P41NOXB
P41NOXC
regulatory protein P41NOX
SH3 and PX domain-containing protein 5
SH3PXD5NADPH oxidase regulatory protein
SNX28
Background
NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Co-transfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5-P2, PtdIns 5-P and PtdIns 4-P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 co-localizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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