![Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Western-Blot-NBP2-02060-img0017.jpg "Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI4D9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.82 mg/ml |
| Immunogen | Human recombinant protein fragment correspongding to amino acids 184-385 of human NONO(NP_031389) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NONO |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 54.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.82 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-02060 | Applications | Species |
|---|---|---|
| Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01 |
Secondary Antibodies |
Isotype Controls |
Research Areas for NONO Antibody (NBP2-02060)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: NONO Antibody (4D9) [NBP2-02060] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunocytochemistry-Immunofluorescence-NBP2-02060-img0016.jpg "Immunocytochemistry/Immunofluorescence: NONO Antibody (4D9) [NBP2-02060] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.")
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0015.jpg "Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.")
![Flow Cytometry: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NONO antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Flow-Cytometry-NBP2-02060-img0001.jpg "Flow Cytometry: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NONO antibody, and then analyzed by flow cytometry.")
![Western Blot: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Western-Blot-NBP2-02060-img0002.jpg "Western Blot: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.")
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0003.jpg "Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0004.jpg "Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0006.jpg "Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0007.jpg "Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0014.jpg "Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.")
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