![Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody [NBP3-23432] - Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23432_rabbit-non-muscle-myosin-iia-p-ser1943-pab-19920231856938.jpg "Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody [NBP3-23432] - Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).")
| Reactivity | Hu, Mu, Rt, ChSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Phospho-non-muscle Myosin IIA (Ser-1943) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse non-muscle Myosin IIA, but is not found in non-muscle Myosin IIB and is not well conserved in non-muscle Myosin IIC. [UniProt# P35579] |
| Modification | p Ser1943 |
| Specificity | This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier). The antibody detects a 200 kDa* protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells. This reactivity is removed after treatment with lambda phosphatase. The antibody also detects NMHC-IIA (Ser-1943) phosphorylation in chick Fibroblasts. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MYH9 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 200 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | MYH9 |
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