![Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-GT246-Western-Blot-NBP3-13591-img0004.jpg "Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | GT246 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human non-muscle heavy chain 10 Myosin. The exact sequence is proprietary. |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MYH10 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 229 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MYH10 |
![Immunocytochemistry/Immunofluorescence: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: non-muscle heavy chain 10 Myosin protein stained by non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-GT246-Immunocytochemistry-Immunofluorescence-NBP3-13591-img0005.jpg "Immunocytochemistry/Immunofluorescence: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: non-muscle heavy chain 10 Myosin protein stained by non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Frozen: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: non-muscle heavy chain 10 Myosin stained by non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-GT246-Immunohistochemistry-Frozen-NBP3-13591-img0007.jpg "Immunohistochemistry-Frozen: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: non-muscle heavy chain 10 Myosin stained by non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min")
![Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-GT246-Western-Blot-NBP3-13591-img0001.jpg "Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-GT246-Western-Blot-NBP3-13591-img0002.jpg "Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000.](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-GT246-Western-Blot-NBP3-13591-img0003.jpg "Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000.")
![Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/non-muscle-heavy-chain-10-Myosin-Antibody-GT246-Immunohistochemistry-Paraffin-NBP3-13591-img0006.jpg "Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunohistochemistry_22971.jpg)
![Immunohistochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunohistochemistry_22972.jpg)
![Immunocytochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunocytochemistry_9890.jpg)
