![Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP1-31733-img0022.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Ca, ChSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human non-muscle Myosin IIA. The exact sequence is proprietary. |
| Predicted Species | Bovine (99%), Chicken (94%), Canine (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MYH9 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 227 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Publication using NBP1-31733 | Applications | Species |
|---|---|---|
| Yoon Y, Go G, Yun C et al. Knockdown of CK2 alpha reduces P-cresol-induced fibrosis in human renal proximal tubule epithelial cells via the downregulation of profilin-1 Int. J. Med. Sci. 2020-10-28 [PMID: 33162813] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for non-muscle Myosin IIA Antibody (NBP1-31733)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-31733] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 antibody [N1-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31733-img0020.jpg "Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-31733] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 antibody [N1-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-31733-img0015.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug Rat2 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP1-31733-img0017.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug Rat2 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000")
![Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP1-31733-img0019.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-31733-img0014.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP1-31733] - Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1-2], N-term. Western blot analysis was performed using MYH9 antibody [N1-2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunoprecipitation-NBP1-31733-img0013.jpg "Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP1-31733] - Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1-2], N-term. Western blot analysis was performed using MYH9 antibody [N1-2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31733_rabbit-polyclonal-non-muscle-myosin-iia-antibody-1610202419405784.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunohistochemistry_22971.jpg)
![Immunohistochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunohistochemistry_22972.jpg)
![Immunocytochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]](https://images.novusbio.com/images2/Myosin_Heavy_Chain_MAB4470_Immunocytochemistry_9890.jpg)
![Western Blot Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Western_Blot_19121.jpg)
![Immunohistochemistry Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Immunohistochemistry_19158.jpg)
![Immunohistochemistry Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nephrin_AF3159_Immunohistochemistry_6775.jpg)
![Immunohistochemistry Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nephrin_AF3159_Immunohistochemistry_10004.jpg)
![Immunocytochemistry/ Immunofluorescence Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images/af3159_mouse-nephrin-affinity-purified-polyclonal-ab-41202410484365.jpg)
![Western Blot Uromodulin Antibody [Unconjugated]](https://images.novusbio.com/images2/Uromodulin_AF5175_Western_Blot_5648.jpg)
![Immunohistochemistry Uromodulin Antibody [Unconjugated]](https://images.novusbio.com/images2/Uromodulin_AF5175_Immunohistochemistry_12099.jpg)
![Immunocytochemistry/ Immunofluorescence Uromodulin Antibody [Unconjugated]](https://images.novusbio.com/images/af5175_mouse-uromodulin-affinity-purified-polyclonal-ab-41202410481117.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")