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Noggin Antibody (OTI1H8) - Azide and BSA Free

Images

 
Western Blot: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - Noggingin antibody (1H8) at 1:40000 + Recombinant human Noggingin.
Immunocytochemistry/ Immunofluorescence: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Noggin.
Immunohistochemistry: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - Staining of paraffin-embedded Human lung tissue using anti-Noggin mouse monoclonal antibody.
Western Blot: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.
Western Blot: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Noggin (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - Staining of paraffin-embedded Human Ovary tissue using anti-Noggin mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Noggin Antibody (OTI1H8) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NOG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Western Blot 1:20000-40000
Theoretical MW
23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Noggin Antibody (OTI1H8) - Azide and BSA Free

  • NOG
  • Noggin
  • SYM1
  • symphalangism 1 (proximal)
  • synostoses (multiple) syndrome 1
  • SYNS1
  • SYNS1A

Background

Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NOG