Novus Biologicals products are now on bio-techne.com

NKp44/NCR2 Antibody (253415) [Alexa Fluor® 350]

Images

 
There are currently no images for NKp44/NCR2 Antibody (FAB22491U).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
253415
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

NKp44/NCR2 Antibody (253415) [Alexa Fluor® 350] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human NKp44/NCR2
Gln22-Pro190
Accession # CAB52289
Specificity
Detects human NKp44/NCR2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp46, rhNKp80, or recombinant mouse NKp46 is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NCR2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp44/NCR2 Antibody (253415) [Alexa Fluor® 350]

  • CD336 antigen
  • CD336
  • LY95
  • LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44)
  • lymphocyte antigen 95 (activating NK-receptor; NK-p44)
  • Lymphocyte antigen 95 homolog
  • natural cytotoxicity triggering receptor 2
  • Natural killer cell p44-related protein
  • NCR2
  • NK cell activating receptor (NKp44)
  • NK cell-activating receptor
  • NKp44
  • NK-p44dJ149M18.1

Background

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is upregulated upon activation with IL-2. Activation-induced expression occurs in the CD56dim CD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56bright CD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
  5. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  6. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2225
Species: Mu
Applications: AgAct, CyTOF-ready, Flow, IHC, WB
1849-NK
Species: Hu
Applications: BA
MAB139
Species: Hu
Applications: CostimT, CyTOF-ready, Flow, Neut, WB
202-IL
Species: Hu
Applications: BA
AF2408
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, KO, Simple Western, WB
AF1597
Species: Hu
Applications: Block, WB
NBP1-85313
Species: Hu
Applications: IHC, IHC-P, WB
7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
4325-FC
Species: Hu
Applications: Bind
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
MAB1058
Species: Hu
Applications: Block, CyTOF-ready, Flow
247-ILB
Species: Hu
Applications: BA
782-IL/CF
Species: Hu
Applications: BA
MAB1059
Species: Hu
Applications: CyTOF-ready, Flow
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NB600-1441
Species: Ca, Hu, Mu, Po
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
MAB1225
Species: Hu
Applications: Block, CyTOF-reported, Flow
FAB22491U
Species: Hu
Applications: Flow

Publications for NKp44/NCR2 Antibody (FAB22491U) (0)

There are no publications for NKp44/NCR2 Antibody (FAB22491U).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NKp44/NCR2 Antibody (FAB22491U) (0)

There are no reviews for NKp44/NCR2 Antibody (FAB22491U). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NKp44/NCR2 Antibody (FAB22491U) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NKp44/NCR2 Antibody (253415) [Alexa Fluor® 350] and receive a gift card or discount.

Bioinformatics

Gene Symbol NCR2
Uniprot