NKG2C/CD159c/KLRC2 Antibody (134522) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8⁺ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8⁺ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C⁺ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12). MAB1381 (clone 134522) displays potent agonistic activity and also blocks the binding of the NKG2C/CD94 heterodimer to HLA-E tetramers (13-15).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Marmoset.

We have publications tested in 6 applications: Affinity Chromatography, CyTof, ELISA Capture, Flow Cytometry, Immunoprecipitation, Neutralization.

Showing Publications 1 - 10 of 18. Show All 18 Publications. Collapse Publications.

Publications using MAB1381	Applications	Species
Alice Mac Donald, Delphine Guipouy, William Lemieux, Mario Harvey, Louis-Jean Bordeleau, David Guay et al. KLRC1 knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumors Frontiers in Immunology 8/21/2023 [PMID: 37675109]
Y Maeda, H Wada, D Sugiyama, T Saito, T Irie, K Itahashi, K Minoura, S Suzuki, T Kojima, K Kakimi, J Nakajima, T Funakoshi, S Iida, M Oka, T Shimamura, T Doi, Y Doki, E Nakayama, R Ueda, H Nishikawa Depletion of central memory CD8+ T cells might impede the antitumor therapeutic effect of Mogamulizumab Nature Communications, 2021-12-14;12(1):7280. 2021-12-14 [PMID: 34907192] (Neutralization, Human)	Neutralization	Human
GT Hò, AA Celik, T Huyton, W Hiemisch, R Blasczyk, GS Simper, C Bade-Doedi NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain Int J Mol Sci, 2020-06-19;21(12):. 2020-06-19 [PMID: 32575403] (Affinity Chromatography, ELISA Capture, Human)	Affinity Chromatography, ELISA Capture	Human
Marcel Costa-García, Michelle Ataya, Manuela Moraru, Carlos Vilches, Miguel López-Botet, Aura Muntasell Human Cytomegalovirus Antigen Presentation by HLA-DR+ NKG2C+ Adaptive NK Cells Specifically Activates Polyfunctional Effector Memory CD4+ T Lymphocytes Frontiers in Immunology 4/3/2019 [PMID: 31001281]
WC Pump, T Kraemer, T Huyton, GT Hò, R Blasczyk, C Bade-Doedi Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8? T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia Int J Mol Sci, 2019-03-22;20(6):. 2019-03-22 [PMID: 30909402] (Immunoprecipitation, Human)	Immunoprecipitation	Human
T Fali, L Papagno, C Bayard, Y Mouloud, J Boddaert, D Sauce, V Appay New Insights into Lymphocyte Differentiation and Aging from Telomere Length and Telomerase Activity Measurements J. Immunol., 2019-02-08;0(0):. 2/8/2019 [PMID: 30737273]
W Ye, M Chew, J Hou, F Lai, SJ Leopold, HL Loo, A Ghose, AK Dutta, Q Chen, EE Ooi, NJ White, AM Dondorp, P Preiser, J Chen Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway PLoS Pathog., 2018-10-04;14(10):e1007298. 2018-10-04 [PMID: 30286211] (Flow Cytometry, Human)	Flow Cytometry	Human
CY Chiu, C Sánchez-Sa, J Bouquet, T Li, S Yagi, M Tamhankar, VL Hodara, LM Parodi, S Somasekar, G Yu, LD Giavedoni, S Tardif, J Patterson Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection Sci Rep, 2017-12-07;7(1):17126. 2017-12-07 [PMID: 29215081] (Flow Cytometry, Marmoset)	Flow Cytometry	Marmoset
Vassilios Lougaris NFKB1 regulates human NK cell maturation and effector functions Clin. Immunol, 2016-12-03;0(0):. 2016-12-03 [PMID: 27923702] (Flow Cytometry, Human)	Flow Cytometry	Human
Dolores Redondo-Pa Adaptive NKG2C+ NK Cell Response and the Risk of Cytomegalovirus Infection in Kidney Transplant Recipients J. Immunol, 2016-12-02;0(0):. 2016-12-02 [PMID: 27913630] (Flow Cytometry, Human)	Flow Cytometry	Human
Alexander W. Kay, Dara M. Strauss-Albee, Catherine A. Blish Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells Natural Killer Cells 1/25/2017 [PMID: 27177653]
Anton O, Vielkind S, Peterson M, Tagaya Y, Long E NK Cell Proliferation Induced by IL-15 Transpresentation Is Negatively Regulated by Inhibitory Receptors. J Immunol, 2015-10-09;195(10):4810-21. 2015-10-09 [PMID: 26453750] (Flow Cytometry, Human)	Flow Cytometry	Human
Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation. J Immunol, 2015-09-28;195(9):4524-36. 2015-09-28 [PMID: 26416275] (CyTof, Human)	CyTof	Human
Costa-Garcia M, Vera A, Moraru M, Vilches C, Lopez-Botet M, Muntasell A Antibody-mediated response of NKG2Cbright NK cells against human cytomegalovirus. J Immunol, 2015-02-09;194(6):2715-24. 2015-02-09 [PMID: 25667418] (Flow Cytometry, Human)	Flow Cytometry	Human
Heatley , Susan L, Pietra , Gabriell, Lin , Jie, Widjaja , Jacqueli, Harpur , Christop, Lester , Sue, Rossjohn , Jamie, Szer , Jeff, Schwarer , Anthony, Bradstock , Kenneth, Bardy , Peter G, Mingari , Maria Cr, Moretta , Lorenzo, Sullivan , Lucy C, Brooks , Andrew G Polymorphism in human cytomegalovirus UL40 impacts on recognition of human leukocyte antigen-E (HLA-E) by natural killer cells. J Biol Chem, 2013-01-18;288(12):8679-90. 2013-01-18 [PMID: 23335510] (Flow Cytometry, Human)	Flow Cytometry	Human
Isabelle Riederer, Wolfgang Sievert, Günther Eissner, Michael Molls, Gabriele Multhoff Irradiation-Induced Up-Regulation of HLA-E on Macrovascular Endothelial Cells Confers Protection against Killing by Activated Natural Killer Cells PLoS ONE 12/16/2010 [PMID: 21179573]
Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components. Blood, 2008-01-11;111(6):3155-62. 2008-01-11 [PMID: 18192509] (Neutralization, Human)	Neutralization	Human
Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood, 2007-04-01;109(7):2806-14. 2007-04-01 [PMID: 17179229] (Neutralization, Human)	Neutralization	Human
Show All 18 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	Flow	Other	02/10/2015	View Summary Application Flow Cytometry Sample Tested See PMID 24660852 Species Other

Array MAB1381

• NKG2C/CD159c/KLRC2 Antibodies
• NKG2C/CD159c/KLRC2 Lysate
• NKG2C/CD159c/KLRC2 Proteins
• NKG2C/CD159c/KLRC2 Proteins and Enzyme