KAT4/TBP Associated Factor 1 Antibody Summary
Description |
This affinity purified antibody is directed against human KAT4/TBP Associated Factor 1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
Immunogen |
This affinity purified KAT4/TBP Associated Factor 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human KAT4/TBP Associated Factor 1. (Uniprot: P21675) |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
TAF1 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Chromatin Immunoprecipitation (ChIP) 1:10-1:500
- ELISA 1:150000-1:300000
- Immunohistochemistry 10ug/ml
- Immunohistochemistry-Paraffin 10 ug/ml
- Immunoprecipitation 1:10 - 1:500
- Western Blot 1:100-1:2000
|
Application Notes |
This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, ChIP, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 213 kDa in size corresponding to TAF1 protein by western blotting in the appropriate cell lysate or extract. |
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with KAT4/TBP Associated Factor 1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Packaging, Storage & Formulations
Storage |
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Preservative |
0.01% Sodium Azide |
Purity |
Immunogen affinity purified |
Alternate Names for KAT4/TBP Associated Factor 1 Antibody
Background
Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53. TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18% of mammalian genes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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