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IRF6 Antibody

 (8 Publications)
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Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).
Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: IRF6 Antibody [NBP1-51911] - (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, ChIP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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IRF6 Antibody Summary

Immunogen
Peptide with sequence C-TPSMQLPPALPPQ corresponding to C-Terminus according to NP_006138.1.
Epitope
C-TPSMQLPPALPPQ
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRF6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 0.6 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 53.1 kDa band according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human kidney shows strong nuclear staining in DCT and glomerulus. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 21807998).
Publications
Read Publications using
NBP1-51911 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25358290).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRF6 Antibody

  • interferon regulatory factor 6
  • IRF6
  • IRF-6
  • LPS
  • OFC6
  • OFC6PPS
  • PIT
  • PPS
  • Van der Woude syndrome
  • VWS
  • VWS1

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF6 Antibody (NBP1-51911)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Block/Neutralize, ChIP, Chemotaxis, IP, WB, Western Blot.


Filter By Application
Block/Neutralize
(1)
ChIP
(1)
Chemotaxis
(1)
IP
(1)
WB
(2)
Western Blot
(1)
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Mouse
(2)
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Showing Publications 1 - 8 of 8.
Publications using NBP1-51911 Applications Species
Kim IK, Diamond M, Yuan S et al. Plasticity-induced repression of Irf6 underlies acquired resistance to cancer immunotherapy Research Square 2023-06-02 [PMID: 37398248] (Western Blot, Block/Neutralize) Western Blot, Block/Neutralize
Boudra R, Patenall BL, King S et al. PRMT1 Inhibition Selectively Targets BNC1-Dependent Proliferation, but not Migration in Squamous Cell Carcinoma bioRxiv : the preprint server for biology 2023-03-27 [PMID: 37034732] (ChIP) ChIP
Li C, Ying W, Huang Z et al. IRF6 regulates alternative activation by suppressing PPARg in male murine macrophages. Endocrinology. 2017-06-22 [PMID: 28645193]
Huang R, Hu Z et al. The Transcription Factor IRF6 Co-Represses PPAR gamma-Mediated Cytoprotection in Ischemic Cerebrovascular Endothelial Cells. Sci Rep 2017-05-19 [PMID: 28526834] (IP, Mouse) IP Mouse
Hu L, Liu J, Li Z et al. TGFB3 Regulates Periderm Removal through delta-np63 in the Developing Palate J. Cell. Physiol. 2014-10-30 [PMID: 25358290] (WB, Mouse)

Details:
IRF6 antibody used for WB on lysates of medial edge epithelial cells collected from WT mouse at 14.25 dpc subjected to exogenous recombinant rTGF beta 3 (5ng/mL) treatment. Figure 6 i and ii shows the time and concentration dependent response of cells to rTGF beta 3. 		WB Mouse
Botti E, Spallone G, Moretti F et al. Developmental factor IRF6 exhibits tumor suppressor activity in squamous cell carcinomas. Proc Natl Acad Sci U S A 2011-08-16 [PMID: 21807998] (Chemotaxis) Chemotaxis
Moretti F, Marinari B, Lo Iacono N et al. A regulatory feedback loop involving p63 and IRF6 links the pathogenesis of 2 genetically different human ectodermal dysplasias. J Clin Invest 2010-04-26 [PMID: 20424325] (WB) WB
Kondo S, Schutte BC, Richardson RJ et al. Mutations in IRF6 cause Van der Woude and popliteal pterygium syndromes. Nat Genet 2002-10-01 [PMID: 12219090]

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Video Protocols

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Bioinformatics

Gene Symbol IRF6
Entrez
Uniprot