![Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of NUR77 on rat brain tissue lysates using anti-NUR77 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Western-Blot-NBP2-66980-img0008.jpg "Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of NUR77 on rat brain tissue lysates using anti-NUR77 antibody at 1/500 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | JM59-11 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human NGFI-B alpha/Nur77/NR4A1 aa 10-49 / 598. (SwissProt: P22736 Human; SwissProt: P12813 Mouse; SwissProt: P22829 Rat) |
| Localization | Cytoplasm. Nucleus. Nucleus membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NR4A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunocytochemistry__Immunofluorescence_21634.jpg)
![Immunohistochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunohistochemistry_9938.jpg)
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![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Human | 11/26/2021 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for NGFI-B alpha/Nur77/NR4A1 Antibody (NBP2-66980)Find related products by research area.
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Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NR4A1 |
![Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Staining NUR77 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunocytochemistry-Immunofluorescence-NBP2-66980-img0009.jpg "Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Staining NUR77 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Human lymph node tissue stained with NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunohistochemistry-Paraffin-NBP2-66980-img0010.jpg "Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Human lymph node tissue stained with NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11). IHC-P image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Staining NUR77 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunocytochemistry-Immunofluorescence-NBP2-66980-img0001.jpg "Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Staining NUR77 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Staining NUR77 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunocytochemistry-Immunofluorescence-NBP2-66980-img0002.jpg "Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Staining NUR77 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded human breast tissue using anti-NUR77 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunohistochemistry-Paraffin-NBP2-66980-img0003.jpg "Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded human breast tissue using anti-NUR77 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded human colon cancer tissue using anti-NUR77 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunohistochemistry-Paraffin-NBP2-66980-img0004.jpg "Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded human colon cancer tissue using anti-NUR77 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded human liver tissue using anti-NUR77 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunohistochemistry-Paraffin-NBP2-66980-img0005.jpg "Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded human liver tissue using anti-NUR77 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded mouse ovarian using anti-NUR77 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunohistochemistry-Paraffin-NBP2-66980-img0006.jpg "Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded mouse ovarian using anti-NUR77 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded mouse testis tissue using anti-NUR77 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-JM59-11-Immunohistochemistry-Paraffin-NBP2-66980-img0007.jpg "Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody (JM59-11) [NBP2-66980] - Analysis of paraffin-embedded mouse testis tissue using anti-NUR77 antibody. Counter stained with hematoxylin.")
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![Immunohistochemistry Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2156_human-mouse-nurr1-ngfi-b-beta-nr4a2-affinity-purified-pab-immunohistochemistry-10122021111228.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
