![Western Blot: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Azide-and-BSA-Free-Western-Blot-NBP2-33062-img0001.jpg "Western Blot: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).")
| Reactivity | Hu, Mu, Rt, Po, ChSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | RT97 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29435). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Triton-X 100 insoluble protein fraction of rat brain (Uniprot: P12036) |
| Localization | Cytoplasmic |
| Marker | Neuronal Marker |
| Specificity | This monoclonal antibody reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NEFH |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 200 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
| Publication using NBP2-33062 | Applications | Species |
|---|---|---|
| Reincke SM, von Wardenburg N, Homeyer MA et al. Chimeric autoantibody receptor T cells deplete NMDA receptor-specific B cells Cell 2023-10-26 [PMID: 37918394] (IHC-P, Mouse) Details: 1:200 dilution |
IHC-P | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for NF-H Antibody (NBP2-33062)Find related products by research area.
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Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera... Read full blog post. |
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| Gene Symbol | NEFH |
![Immunohistochemistry-Paraffin: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33062-img0003.jpg "Immunohistochemistry-Paraffin: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (RT97).")
![Immunohistochemistry-Paraffin: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33062-img0002.jpg "Immunohistochemistry-Paraffin: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-H Antibody (RT97).")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")