![Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.The ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0007.jpg "Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.The ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary. |
| Localization | Cytoplasm; perinuclear region; Golgi apparatus; cytoskeleton |
| Predicted Species | Chimpanzee (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CALCOCO2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
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Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI.Scale bar= 10 um.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19499-img0010.jpg "Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI.Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499] - H441 xenograft, using NDP52 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP2-19499-img0003.jpg "Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499] - H441 xenograft, using NDP52 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: NDP52 Antibody [NBP2-19499] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0002.jpg "Western Blot: NDP52 Antibody [NBP2-19499] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0006.jpg "Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunoprecipitation: NDP52 Antibody [NBP2-19499] - Jurkat whole cell extracts using 5 ug of NDP52 antibody. Western blot analysis was performed using NDP52 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunoprecipitation-NBP2-19499-img0009.jpg "Immunoprecipitation: NDP52 Antibody [NBP2-19499] - Jurkat whole cell extracts using 5 ug of NDP52 antibody. Western blot analysis was performed using NDP52 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot: NDP52 Antibody [NBP2-19499] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (NBP2-19499) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19499_rabbit-polyclonal-ndp52-antibody-910202420134026.jpg "Western Blot: NDP52 Antibody [NBP2-19499] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (NBP2-19499) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot Galectin-8 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin8_AF1305_Western_Blot_16880.jpg)
![Simple Western Galectin-8 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin8_AF1305_Simple_Western_18662.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")