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![Western Blot: NDP52 Antibody [H00010241-B01P] - Hypothalamic autophagy at day 17. (c) Representative immunoblots (from 3 individuals/group) and expression profile (n = 6/group) of BNIP3 and NDP52 at d17 in the hypothalamus of Control mice (open bars) or mice with limitation of food access (LFA, grey bars) or activity-based anorexia (ABA, black bars). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29740148) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0007.jpg "Western Blot: NDP52 Antibody [H00010241-B01P] - Hypothalamic autophagy at day 17. (c) Representative immunoblots (from 3 individuals/group) and expression profile (n = 6/group) of BNIP3 and NDP52 at d17 in the hypothalamus of Control mice (open bars) or mice with limitation of food access (LFA, grey bars) or activity-based anorexia (ABA, black bars). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29740148) licensed under a CC-BY license.")
![Western Blot: NDP52 Antibody [H00010241-B01P] - CALCOCO2 MaxPab polyclonal antibody. Western Blot analysis of CALCOCO2 expression in human pancreas.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0003.jpg)
![Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in human liver.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0004.jpg)
![Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in transfected 293T cell line by CALCOCO2 polyclonal antibody. Lane 1: CALCOCO2 transfected lysate(49.06 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0006.jpg)
![Western Blot: NDP52 Antibody [H00010241-B01P] - Analysis of CALCOCO2 expression in human pancreas.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-H00010241-B01P-img0005.jpg)
![Western Blot: NDP52 Antibody [H00010241-B01P] - Hypothalamic autophagy at day 17. (a) Representative immunoblots (n = 1/group) & expression profile (n = 6/group) of LC3I, LC3II at d17 (Supplemental Fig. S8) & (b) representative immunoblots (n = 1/group) & expression profile (n = 6/group) of DNM1 at d17 in the hypothalamus of Control mice (open bars) or mice with limitation of food access (LFA, grey bars) or activity-based anorexia (ABA, black bars). (c) Representative immunoblots (from 3 individuals/group) & expression profile (n = 6/group) of BNIP3 & NDP52 at d17 in the hypothalamus of Control mice (open bars) or mice with limitation of food access (LFA, grey bars) or activity-based anorexia (ABA, black bars). *p < 0.05 vs Control. #p < 0.05 vs LFA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29740148), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010241-b01p_mouse-polyclonal-ndp52-antibody-310202415304229.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining in human testis and pancreas tissues using anti-CALCOCO2 antibody. Corresponding CALCOCO2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining in human testis and pancreas tissues using anti-CALCOCO2 antibody. Corresponding CALCOCO2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: NDP52 Antibody [NBP1-87872] - Analysis using Anti-CALCOCO2 antibody NBP1-87872 (A) shows similar pattern to independent antibody NBP1-87874 (B).](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP1-87872-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP1-87872] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87872-img0012.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0023.jpg)
![Western Blot: NDP52 Antibody [NBP1-87872] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP1-87872-img0013.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0009.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0018.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87872] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87872-img0021.jpg)
![Western Blot: NDP52 Antibody [NBP1-87874] - Analysis using Anti-CALCOCO2 antibody NBP1-87874 (A) shows similar pattern to independent antibody NBP1-87872 (B).](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP1-87874-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NDP52 Antibody [NBP1-87874] - Analysis using Anti-CALCOCO2 antibody NBP1-87874 (A) shows similar pattern to independent antibody NBP1-87872 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP1-87874] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87874-img0006.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0020.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Analysis in human testis and pancreas tissues. Corresponding NDP52 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human gastrointestinal, kidney, pancreas and testis using Anti-NDP52 antibody NBP1-87874 (A) shows similar protein distribution across tissues to independent antibody NBP1-87873 (B).](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0015.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0017.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0018.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87874] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87874-img0019.jpg)
![Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.The ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.The ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI.Scale bar= 10 um.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19499-img0010.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499] - H441 xenograft, using NDP52 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP2-19499-img0003.jpg)
![Western Blot: NDP52 Antibody [NBP2-19499] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP2-19499-img0006.jpg)
![Immunoprecipitation: NDP52 Antibody [NBP2-19499] - Jurkat whole cell extracts using 5 ug of NDP52 antibody. Western blot analysis was performed using NDP52 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunoprecipitation-NBP2-19499-img0009.jpg)
![Western Blot: NDP52 Antibody [NBP2-19499] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (NBP2-19499) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19499_rabbit-polyclonal-ndp52-antibody-910202420134026.jpg)
![Western Blot: NDP52 Antibody (422) [NBP2-43696] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [422].](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NDP52 Antibody (422) [NBP2-43696] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [422].")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - NDP52 antibody (422) detects NDP52 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody (422) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Immunocytochemistry-Immunofluorescence-NBP2-43696-img0012.jpg)
![Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of A. 30 ug Huh7 whole cell lysate/extract B. 30 ug Hep3B whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [422] dilution: 1:1000](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0001.jpg)
![Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [422] dilution: 1:1000.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs. HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Western-Blot-NBP2-43696-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [422]. Red: Phalloidin, a F-actin marker.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Immunocytochemistry-Immunofluorescence-NBP2-43696-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - Analysis of Samples: HepG2 cells mock (left) and treated with 3 uM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec. Green: NDP52 protein stained by NDP52 antibody [422]. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Immunocytochemistry-Immunofluorescence-NBP2-43696-img0011.jpg)
![Immunoprecipitation: NDP52 Antibody (422) [NBP2-43696] - Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 ug of NDP52 antibody [422]. Western blot analysis was performed using NDP52 antibody [422]. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/NDP52-Antibody-422-Immunoprecipitation-NBP2-43696-img0006.jpg)
![Western Blot: NDP52 Antibody (4H5) [NBP2-03246] Analysis of extracts (35ug) from 9 different cell lines by using anti-NDP52 monoclonal antibody.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Western-Blot-NBP2-03246-img0006.jpg "Western Blot: NDP52 Antibody (4H5) [NBP2-03246] Analysis of extracts (35ug) from 9 different cell lines by using anti-NDP52 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: NDP52 Antibody (4H5) [NBP2-03246] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDP52.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-03246-img0005.jpg)
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDP52 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0003.jpg)
![Western Blot: NDP52 Antibody (4H5) [NBP2-03246] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDP52 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDP52.](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Western-Blot-NBP2-03246-img0004.jpg)
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - Analysis of Hela cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0001.jpg)
![Flow Cytometry: NDP52 Antibody (4H5) [NBP2-03246] - Analysis of Jurkat cells, using anti-NDP52 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NDP52-Antibody-4H5-Flow-Cytometry-NBP2-03246-img0002.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Analysis in human testis and pancreas tissues. Corresponding NDP52 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Analysis in human testis and pancreas tissues. Corresponding NDP52 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human gastrointestinal, kidney, pancreas and testis using Anti-NDP52 antibody NBP1-87873 (A) shows similar protein distribution across tissues to independent antibody NBP1-87874 (B).](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0014.jpg)
![Western Blot: NDP52 Antibody [NBP1-87873] - Analysis in control (vector only transfected HEK293T lysate) and CALCOCO2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NDP52-Antibody-Western-Blot-NBP1-87873-img0009.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0018.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0015.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0016.jpg)
![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP1-87873] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP1-87873-img0017.jpg)
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT1813].](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT1813].")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: NDP52 Antibody (1813) [NBP2-43588] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [1813]. Red: Phalloidin, a F-actin marker.](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Immunocytochemistry-Immunofluorescence-NBP2-43588-img0006.jpg)
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of A. 30 ug Huh7 whole cell lysate/extract B. 30 ug Hep3B whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [1813] dilution: 1:1000](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0001.jpg)
![Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [1813] dilution: 1:1000.](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. The ACTB was used as an internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDP52 Antibody (1813) [NBP2-43588] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).](http://images.novusbio.com/fullsize/NDP52-Antibody-1813-Western-Blot-NBP2-43588-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: NDP52 Antibody [NBP3-38417] - Immunofluorescence analysis of PC-12 cells using NDP52 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38417_rabbit-ndp52-pab-1012202411343373.jpg "Immunocytochemistry/ Immunofluorescence: NDP52 Antibody [NBP3-38417] - Immunofluorescence analysis of PC-12 cells using NDP52 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: NDP52 Antibody [NBP3-38417] - Western blot analysis of lysates from mouse pancreas, using NDP52 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38417_rabbit-ndp52-pab-1012202411421036.jpg)
![Western Blot: NDP52 Antibody (PSH02-33) [NBP3-32636] - Western blot analysis of NDP52 on different lysates with Rabbit anti-NDP52 antibody (NBP3-32636) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Huh7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Saos-2 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32636) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32636_rabbit-ndp52-mab-psh02-33-278202415212481.jpg "Western Blot: NDP52 Antibody (PSH02-33) [NBP3-32636] - Western blot analysis of NDP52 on different lysates with Rabbit anti-NDP52 antibody (NBP3-32636) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Huh7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Saos-2 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32636) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: NDP52 Antibody (PSH02-33) [NBP3-32636] - Flow cytometric analysis of HeLa cells labeling NDP52. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32636, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32636_rabbit-ndp52-mab-psh02-33-29202416453247.jpg)
![Western Blot: NDP52 Antibody (5O8Y6) [NBP3-33327] - Western blot analysis of various lysates using NDP52 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33327_rabbit-ndp52-mab-5o8y6-912202415121386.jpg "Western Blot: NDP52 Antibody (5O8Y6) [NBP3-33327] - Western blot analysis of various lysates using NDP52 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")